›› 2007, Vol. 34 ›› Issue (5): 57-59.

• 遗传繁育 • 上一篇    下一篇

EGF和IGF-1对水牛卵母细胞体外成熟培养的影响

王晓丽,农垚峰,冯贵雪,石德顺,韦精卫,周红英
  

  1. 广西大学动物科学技术学院,南宁 530005
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-20 发布日期:2007-05-20

Effects of EGF and IGF-1 on Maturation of Buffalo Oocytes in Vitro

WANG Xiaoli,NONG Yaofeng,FENG Guixue,SHI Deshun,WEI Jingwei, ZHOU Hongying
  

  1. College of Animal Science and Technology, Guangxi University, Nanning 530005,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20

摘要: 作者研究了在培养液中添加不同浓度的EGF、IGF-1以及EGF联合IGF-1对水牛卵母细胞体外成熟的影响。结果表明:①添加各种浓度的EGF都可以提高水牛卵母细胞的成熟率,其中50 ng/ml EGF有显著影响(P<0.05)。②10、20 ng/ml的IGF1对水牛卵母细胞体外成熟无显著影响(P>0.05); 30 ng/ml的IGF-1能显著提高水牛卵母细胞体外成熟率(P<0.05)。③添加20 ng/ml EGF+30 ng/ml IGF-1组卵母细胞体外成熟率高于添加30 ng/ml的IGF-1组,显著高于添加20 ng/ml EGF组和对照组(P<0.05)。可见,EGF和IGF-1对水牛卵母细胞体外成熟有协同作用。

关键词: EGF; IGF-1; 卵母细胞; 体外成熟

Abstract: Three experiments were conducted to determine the effect of EGF and IGF1 on maturation of buffalo oocytes in vitro. Oocytes were cultured in oocytes maturation medium with EGF and IGF-1 alone or combinedly at different concentration. The results in experimentⅠshowed that there were significant different between control group and the group with EGF(10,20,30,50 ng/ml) in maturation rate, the group with 50 ng/ml EGF was significantly higher than others(P<0.05). In experiment II the maturation rate of buffalo oocytes was improved significantly in the groups with IGF(30 ng/ml)(P<0.05), but not in the group with 10 ng/ml IGF-1. In experiment III the maturation rate in the group containing 20 ng/ml EGF and 30 ng/ml IGF-1 was significantly higher than 30 ng/ml IGF-1 or 20 ng/ml EGF groups(P<0.05). The results obtained in this study indicate that EGF and IGF-1 have coeffects on maturation of buffalo oocytes in vitro.

Key words: EGF; IGF-1; oocytes; in vitro maturation

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