地黄归芩胶囊对特应性皮炎的治疗效果及作用机制研究

doi:10.16431/j.cnki.1671-7236.2025.05.041

摘要/Abstract

摘要： 【目的】探究地黄归芩胶囊对二硝基氯苯溶液所致大鼠特应性皮炎的治疗效果及作用机制，为宠物临床新兽药研发提供理论依据。【方法】使用2%二硝基氯苯溶液涂抹大鼠背部复制特应性皮炎模型。造模成功后将40只特应性皮炎大鼠随机分为4组，分别为模型对照组及地黄归芩胶囊低(0.1 g/kg)、中(0.2 g/kg)、高(0.4 g/kg)剂量组，每组10只；另选10只健康大鼠作为空白对照组。地黄归芩胶囊各剂量组大鼠按照相应剂量灌胃给药，模型对照组与空白对照组大鼠灌胃饮用水，每次0.2 mL/只，每日1次，连续14 d。按照SCORAD评分标准对大鼠皮损进行评价；记录大鼠搔抓次数评价大鼠瘙痒情况；采用HE染色观察大鼠皮肤组织病理变化情况，利用Baker评分法进行组织病理学评分；通过甲苯胺蓝染色法检测肥大细胞数量；使用Western blotting检测皮肤组织中白细胞介素-31(IL-31)蛋白表达量。【结果】与空白对照组相比，模型对照组大鼠皮损评分显著升高(P＜0.05)，炎症细胞大量浸润，Baker评分显著升高(P＜0.05)。与模型对照组相比，地黄归芩胶囊各剂量组大鼠皮损评分、搔抓次数均显著降低(P＜0.05)；地黄归芩胶囊中、高剂量组大鼠Baker评分、肥大细胞数量均显著减少(P＜0.05)，皮肤组织中IL-31蛋白表达水平显著下调(P＜0.05)。【结论】地黄归芩胶囊能有效缓解特应性皮炎大鼠瘙痒症状，其作用机制与修复皮肤屏障、缓解炎症细胞浸润，减少肥大细胞数量及下调IL-31蛋白表达有关。

关键词: 地黄归芩胶囊; 特应性皮炎; 肥大细胞; 白细胞介素-31

Abstract: 【Objective】 The objective of this experiment was to investigate the therapeutic effect and mechanism of Dihuang Guiqin capsules on atopic dermatitis induced by dinitrochlorobenzene solution in rats,and provide theoretical basis for the research and development of new veterinary drugs for pets.【Method】 The atopic dermatitis model was replicated by applying 2% dinitrochlorobenzene solution on the back of rats.After successful modeling,40 rats with atopic dermatitis were randomly divided into 4 groups:Model control group,Dihuang Guiqin capsules low (0.1 g/kg),medium (0.2 g/kg) and high (0.4 g/kg) dose groups,with 10 rats in each group.10 healthy rats were selected as blank control group.Rats in each dose group were given Dihuang Guiqin capsules by intragastric administration according to the corresponding dose.Rats in model control and blank control groups were given intragastric drinking water,0.2 mL/rat each time,once a day,for 14 consecutive days.The skin lesions of rats were evaluated according to SCORAD.Scratching times of rats were recorded to evaluate the pruritus of rats.The pathological changes of the skin were observed by HE staining,and the histopathological score was performed by Baker score.The number of mast cells was detected by toluidine blue staining.Western blotting was used to detect the expression of interleukin-31 (IL-31) protein in skin tissues.【Result】 Compared with blank control group,the skin lesion score of rats in model control group was significantly increased (P<0.05),a large number of inflammatory cells infiltrated,and the Baker score was significantly increased (P<0.05).Compared with model control group,the skin lesion score and scratching times of rats in each dose group of Dihuang Guiqin capsules were significantly decreased (P<0.05).The Baker score and the number of mast cells in medium and high dose groups of Dihuang Guiqin capsules were significantly decreased (P<0.05),and the expression of IL-31 protein in skin tissue was significantly decreased (P<0.05).【Conclusion】 Dihuang Guiqin capsules could effectively relieve pruritus in rats with atopic dermatitis,and its mechanism related to repairing skin barrier,relieving inflammatory cell infiltration,reducing the number of mast cells and down-regulating IL-31 protein expression.

Key words: Dihuang Guiqin capsules; atopic dermatitis; mast cells; IL-31

中图分类号:

S859.3

秦俊杰, 武超, 黄雪利, 周常喜, 侯晓礁, 王秀敏, 李建喜. 地黄归芩胶囊对特应性皮炎的治疗效果及作用机制研究[J]. 中国畜牧兽医, 2025, 52(5): 2392-2399.

QIN Junjie, WU Chao, HUANG Xueli, ZHOU Changxi, HOU Xiaojiao, WANG Xiumin, LI Jianxi. Study on the Therapeutic Effect and Mechanism of Dihuang Guiqin Capsules on Atopic Dermatitis[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 2392-2399.

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