广东省养鸽场腹泻鸽群沙门菌分离鉴定与耐药性研究

doi:10.16431/j.cnki.1671-7236.2023.12.032

摘要/Abstract

摘要： 【目的】探究广东省某养鸽场沙门菌的流行情况及耐药特征。【方法】本研究从该养鸽场中采集有腹泻表现的病鸽进行剖检,并对样品进行沙门菌的分离鉴定。对分离鉴定为沙门菌的菌株进行血清型鉴定,并采用琼脂稀释法测定分离株对13种抗菌药物的敏感性,再进一步对分离株进行耐药基因以及毒力基因的检测。【结果】从该养鸽场采集的样品中共分离得到沙门菌27株,分离率为67.50%(27/40)。血清型鉴定结果表明,分离株均为鼠伤寒沙门菌。药敏试验结果显示,分离株对萘啶酸、磺胺甲噁唑耐药率为100.00%;对链霉素、四环素、替加环素耐药率为25.93%;对氨苄西林耐药率为11.11%;对氟苯尼考、氯霉素耐药率为3.70%。分离株有37.04%表现为多重耐药,且检出了替加环素的耐药株。耐药基因检测结果显示,分离株可检出共5种耐药基因,其中SulⅡ、tet(A)基因检出率相对较高,分别为29.63%和25.93%;而bla_TEM、floR、cat1基因检出率较低,分别为11.11%、3.70%和3.70%。毒力基因检测结果显示,mogA、mgtC、bcfA、spvR、spvC、stn和sefA基因的检出率为100.00%,sseL基因检出率为96.30%。【结论】本研究成功分离鸽源沙门菌27株,分离株耐药情况严重且检出替加环素耐药株,同时携带多种毒力基因,可能是该鸽群腹泻的主要病原。本研究结果可为养鸽场防控沙门菌提供参考。

关键词: 肉鸽; 沙门菌; 耐药基因; 毒力基因

Abstract: 【Objective】 The aim of this study was to investigate the prevalence and antimicrobial resistance characteristics of Salmonella in a certain pigeon farm in Guangdong province.【Method】 In this study,diseased pigeons with diarrhea were collected from the pigeon farm for dissection,and Salmonella was isolated and identified from the sample.Serotype identification was performed on the strains identified as Salmonella,and the sensitivity of the isolated strains to 13 antimicrobial drugs was determined using agar dilution method.Furthermore,resistance and virulence genes were tested on the isolated strains.【Result】 A total of 27 strains of Salmonella were isolated from the samples collected from the pigeon farm,with a isolation rate of 67.50% (27/40).The serotype identification results showed that all isolated strains were Salmonella Typhimurium.The drug sensitivity test results showed that the resistance rate of the isolated strains to naphthyric acid and sulfamethoxazole was 100.00%.The resistance rate to streptomycin,tetracycline,and tigecycline was 25.93%.The resistance rate to ampicillin was 11.11% and the resistance rate to flufenicol and chloramphenicol was 3.70%.37.04% of the isolated strains showed multiple drug resistance,and resistance to tigecycline was detected.The results of drug resistance gene testing showed that a total of 5 drug resistance genes could be detected in the isolated strains,among which the detection rates of SulⅡ and tet(A) genes were relatively high,with 29.63% and 25.93%,respectively.The detection rates of bla_TEM,floR and cat1 genes were relatively low,with 11.11%,3.70% and 3.70%,respectively.The results of virulence gene testing showed that the detection rate of mogA,mgtC,bcfA,spvR,spvC,stn and sefA genes was 100.00%,and the detection rate of sseL genes was 96.30%.【Conclusion】 This study successfully isolated 27 strains of Salmonella from pigeons,with severe drug resistance and detection of tigecycline resistant strains.They also carried multiple virulence genes,which might be the main cause of diarrhea in this pigeon population.The results of this study could provide reference for the prevention and control of Salmonella in pigeon farms.

Key words: pigeon; Salmonella; antimicrobial resistance; virulence gene

中图分类号:

S852.61

吴灿基, 陈泽銮, 郑泽鑫, 彭钧豪, 何海珊, 梁玉岑, 戴长智, 周洲平, 廖明, 张建民. 广东省养鸽场腹泻鸽群沙门菌分离鉴定与耐药性研究[J]. 中国畜牧兽医, 2023, 50(12): 5104-5113.

WU Canji, CHEN Zeluan, ZHENG Zexin, PENG Junhao, HE Haishan, LIANG Yucen, DAI Changzhi, ZHOU Zhouping, LIAO Ming, ZHANG Jianmin. Isolation,Identification and Antimicrobial Resistance Research of Salmonella from Flock of Diarrhea Pigeons in a Pigeon Farm in Guangdong Province[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5104-5113.

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