关键词: 氨基酸自动分析仪; 饲料原料; 氨基酸含量; 可加性

Abstract: 【Objective】 The objective of this study was to compare the differences in the determination of amino acid contents of feeds using two amino acid analyzers (Biochrom 30⁺,Hitachi L-8900),which was expected to provide a reference for the additivity analysis of amino acid contents of feed measured by different amino acid analyzers.【Method】 A paired experimental design was adapted to compare the differences between two amino acid analyzers for the determination of amino acid contents of energy feeds (corn,brown rice,wheat,barley and paddy) and protein feeds (soybean meal,cottonseed meal,rapeseed meal,corn gluten meal and casein). Meanwhile the additivity of amino acids contents in corn-soybean meal diet were compared when the amino acid were determined with two amino acid analyzers. 【Result】 The contents of 7 amino acids (Lys,Val,Ile,His,Ser,Glu and Ala) in the energy feeds determined by Biochrom 30⁺ were less than those by Hitachi L-8900 (P＜0.05), but the contents of two amino acids (Glu,Cys) in protein feeds determined by Biochrom 30⁺ were higher than those by Hitachi L-8900 (P＜0.05). However,the relative bias of amino acid contents determined by those two amino acid analyzers was less than 3.0％. The differences of amino acid contents of energy feeds determined by two amino acid analyzers were greater than that of protein feeds. The ratios of values for energy feeds and protein feeds determined by Biochrom 30⁺ and Hitachi L-8900 ranged from 94.3％ to 105.0％,and 96.6％ to 102.4％,respectively. The determined values of amino acid content of corn-soybean meal diet by Biochrom 30⁺ and Hitachi L-8900 were 91.4％ to 107.8％ and 99.0％ to 104.3％ of the calculated values,respectively. The relative bias less than 4.0％ was observed between determined and calculated 11(determined by Biochrom 30^＋) and 14(determined by Hitachi L-8900) amino acid contents in corn-soybean meal diet. 【Conclusion】The amino acid contents of energy and protein feed had satisfactory consistency determined by two amino acid analyzers when samples were pretreated with the same condition. Additionally,the additivity of amino acid contents determined by Hitachi L-8900 was greater than that by Biochrom 30^＋.

Key words: amino acid auto analyzer; feed ingredients; amino acid content; additivity