枯草芽孢杆菌YT168-6产抗菌肽sublancin的发酵工艺优化及培养基筛选的研究

doi:10.16431/j.cnki.1671-7236.2021.09.014

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (9): 3232-3241.doi: 10.16431/j.cnki.1671-7236.2021.09.014

枯草芽孢杆菌YT168-6产抗菌肽sublancin的发酵工艺优化及培养基筛选的研究

刘扬科^1,2, 赵天效¹, 卢晓颖^1,2, 李磊^1,2, 成鹏^1,2, 李松建^1,2, 解顺昌^1,2,3

1. 中农颖泰林州生物科园有限公司, 林州 456550;
2. 农业农村部饲用抗生素替代技术重点实验室, 林州 456550;
3. 林州市职业教育中心, 林州 456550

修回日期:2021-03-04 出版日期:2021-09-20 发布日期:2021-09-17
通讯作者: 解顺昌 E-mail:znytxsc@126.com
作者简介:刘扬科(1986-),男,河南信阳人,博士,研究方向:抗菌研究,E-mail:znytlyk@126.com
基金资助:
国家重点研发计划（2017YED501000）

Optimization of Fermentation Technology and Selection of Culture Medium for Antimicrobial Peptide Sublancin Produced by Bacillus subtilis YT168-6

LIU Yangke^1,2, ZHAO Tianxiao¹, LU Xiaoying^1,2, LI Lei^1,2, CHENG Peng^1,2, LI Songjian^1,2, XIE Shunchang^1,2,3

1. Zhongnongyingtai Linzhou Biological Science Park Co., Ltd., Linzhou 456550, China;
2. Key Laboratory of Feed Antibiotic Substitution Technology, Ministry of Agriculture and Rural Affairs, Linzhou 456550, China;
3. Linzhou Vocational Education Center, Linzhou 456550, China

Revised:2021-03-04 Online:2021-09-20 Published:2021-09-17

摘要/Abstract

摘要： 研究旨在探索枯草芽孢杆菌YT168-6产抗菌肽sublancin的最适发酵条件和最适培养基组成，以期提高发酵液中sublancin含量。试验先通过单因素试验筛选出最适发酵条件，确定发酵培养基需要的无机盐、碳源、氮源，再通过正交试验、最陡爬坡试验、Box-Behnken设计的响应面分析方法确定最适发酵培养基组成。结果表明：枯草芽孢杆菌YT168-6产抗菌肽sublancin的最适发酵条件为菌种接种量1.0%、发酵温度37℃、发酵液初始pH 7.0；最适发酵培养基为K₂HPO₄ 5 g/L、KH₂PO₄ 5 g/L、MgSO₄ 10 g/L、可溶性淀粉30.2 g/L、蛋白胨23.6 g/L、玉米浆18.2 g/L；优化后sublancin产量由756 μg/mL提到1 581 μg/mL，是优化前的2.09倍。综上所述，本研究所筛选的最适发酵条件和最适培养基可为枯草芽孢杆菌YT168-6菌株工业化生产sublancin提供技术参考。

关键词: 枯草芽孢杆菌YT168-6; 发酵工艺; sublancin

Abstract: The aim of this study was to research the optimal fermentation conditions and culture medium component for sublancin, an antimicrobial peptide produced by Bacillus subtilis YT168-6, in order to increase the content of sublancin in fermentation broth. A single factor test was used to screen out the optimal fermentation conditions, inorganic salts, carbon and nitrogen sources of fermentation medium. The optimum fermentation medium component was determined by the orthogonal test, the steepest climbing test and the response surface analysis method of Box-Behnken design. The results showed that the optimal fermentation conditions for Bacillus subtilis YT168-6 to produce the antimicrobial peptide sublancin were 1.0% bacterial inoculum, fermentation temperature 37 ℃ and initial pH 7.0, the optimum fermentation medium was K₂HPO₄ 5 g/L, KH₂PO₄ 5 g/L, MgSO₄ 10 g/L, soluble starch 30.2 g/L, peptone 23.6 g/L and corn steep liquor 18.2 g/L. After optimization, the yield of sublancin was increased from 756 μg/mL to 1 581 μg/mL, which was 2.09 times as high as before optimization. In summary, the optimal fermentation conditions and culture medium selected in this research laid the technical foundation for the subsequent industrial production of Bacillus subtilis YT168-6.

Key words: Bacillus subtilis YT168-6; fermentation technology; sublancin

中图分类号:

S816.7

刘扬科, 赵天效, 卢晓颖, 李磊, 成鹏, 李松建, 解顺昌. 枯草芽孢杆菌YT168-6产抗菌肽sublancin的发酵工艺优化及培养基筛选的研究[J]. 中国畜牧兽医, 2021, 48(9): 3232-3241.

LIU Yangke, ZHAO Tianxiao, LU Xiaoying, LI Lei, CHENG Peng, LI Songjian, XIE Shunchang. Optimization of Fermentation Technology and Selection of Culture Medium for Antimicrobial Peptide Sublancin Produced by Bacillus subtilis YT168-6[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3232-3241.

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枯草芽孢杆菌YT168-6产抗菌肽sublancin的发酵工艺优化及培养基筛选的研究

Optimization of Fermentation Technology and Selection of Culture Medium for Antimicrobial Peptide Sublancin Produced by Bacillus subtilis YT168-6

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