饥饿诱发成脂分化C2C12细胞中脂滴形态重塑

doi:10.16431/j.cnki.1671-7236.2020.11.006

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (11): 3460-3466.doi: 10.16431/j.cnki.1671-7236.2020.11.006

饥饿诱发成脂分化C2C12细胞中脂滴形态重塑

刘哲¹, 闫峻², 刘仲昊¹, 穆淑琴²

1. 天津农学院动物科学与动物医学学院, 天津市农业动物繁育与健康养殖重点实验室, 天津 300384;
2. 天津市畜牧兽医研究所, 天津 300381

收稿日期:2020-04-16 出版日期:2020-11-20 发布日期:2020-11-20
通讯作者: 闫峻 E-mail:yjjsxz@163.com
作者简介:刘哲(1993-),女,安徽宿州人,硕士生,研究方向:单胃动物营养,E-mail:1197891058@qq.com
基金资助:
天津市自然科学基金（18JCQNJC15100）；天津市生猪产业技术体系创新团队（IT-TPRS2017005）

Starvation Caused Lipid Droplet Remodeling in Adipogenic Differentiation C2C12 Cells

LIU Zhe¹, YAN Jun², LIU Zhonghao¹, MU Shuqin²

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;
2. Tianjin Institute of Animal Husbandry and Veterinary Medicine, Tianjin 300381, China

Received:2020-04-16 Online:2020-11-20 Published:2020-11-20

摘要/Abstract

摘要： 试验旨在探究短期饥饿处理对成脂分化C2C12细胞中脂滴生长发育的影响，为阐释及利用补偿生长机制奠定基础。通过胰岛素等激素混合物诱导C2C12细胞成脂分化，将成脂分化12 d的C2C12细胞分为两组：对照组（Control），高糖培养24 h；饥饿处理组（FAST），无血清低糖培养24 h。通过BODIPY染色和甘油三酯测定观察脂滴形态变化，并使用Western blotting方法测定脂滴表面结构蛋白perilipin1-5表达量的变化。结果显示，C2C12细胞成脂诱导12 d，可以使细胞达到较好的充脂状态。通过无血清低糖饥饿处理后，成脂分化C2C12细胞中甘油三酯含量极显著下降（P<0.01）；脂滴平均尺寸极显著减小（P<0.01），脂滴平均数量极显著增多（P<0.01）；脂滴表面结构蛋白出现变化，perilipin2、perilipin5表达极显著上调（P<0.01）、perilipin3表达显著上调（P<0.05）。综上，无血清低糖处理可以诱发脂滴形态重塑，改变脂滴表面结构蛋白的表达。

关键词: 成脂分化; C2C12细胞; 脂滴; 脂滴重塑; 饥饿

Abstract: This study was aimed to explore the effect of short-term starvation treatment on the growth and development of lipid droplets in adipogenic differentiation C2C12 cells,provide the foundation for the interpretation and utilization of compensatory growth mechanisms.Adipogenic differentiation C2C12 cells were divided into two groups:Control group,high glucose treatment for 24 h,and starvation treatment group (FAST),serum-free and low-glucose medium starvation treatment for 24 h.The morphological changes of lipid droplets was observed by BODIPY staining and triglyceride determination,and Western blotting was used to determine the change of the expression level of perilipin family protein (perilipin 1-5) on the lipid droplet.The results showed that C2C12 cells induced adipogenesis for 12 days,which could make the cells reach a better fat-filling state.After treatment with serum-free and low-glucose starvation,the levels of triglycerides in adipogenic differentiation C2C12 cells decreased extremely significantly (P<0.01),the size of lipid droplets decreased extremely significantly (P<0.01),and the number of lipid droplets increased extremely significantly (P<0.01).The perilipin protein on the lipid droplets was changed,the expression level of perilipin2 and perilipin5 were extremely significantly up-regulated (P<0.01),and the expression level of perilipin3 was significantly up-regulated (P<0.05).In summary,serum-free and low-glucose starvation could induce the remodeling of lipid droplets and change the expression level of perilipin proteins on the lipid droplets.

Key words: adipogenic differentiation; C2C12 cells; lipid droplets; lipid droplet remodeling; starvation

中图分类号:

Q786

刘哲, 闫峻, 刘仲昊, 穆淑琴. 饥饿诱发成脂分化C2C12细胞中脂滴形态重塑[J]. 中国畜牧兽医, 2020, 47(11): 3460-3466.

LIU Zhe, YAN Jun, LIU Zhonghao, MU Shuqin. Starvation Caused Lipid Droplet Remodeling in Adipogenic Differentiation C2C12 Cells[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3460-3466.

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饥饿诱发成脂分化C2C12细胞中脂滴形态重塑

Starvation Caused Lipid Droplet Remodeling in Adipogenic Differentiation C2C12 Cells

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