肿节风三清颗粒的薄层色谱鉴别和含量测定

doi:10.16431/j.cnki.1671-7236.2019.10.032

摘要/Abstract

摘要： 试验旨在建立肿节风三清颗粒的鉴别和含量测定分析方法，以期为制剂的质量标准提供方法学依据。采用薄层色谱法（TLC）对肿节风三清颗粒中的肿节风、射干、甘草进行定性分析；采用高效液相色谱法（HPLC）对制剂中的异嗪皮啶和迷迭香酸进行含量测定。薄层色谱结果表明，肿节风三清颗粒中的肿节风、射干、甘草在与其对应的标准药材或标准品的相同位置有一致的荧光斑点，而阴性样品无特征荧光斑点。HPLC结果表明，异嗪皮啶在3.795~18.975 μg/mL范围内线性关系良好（R²=0.9995），迷迭香酸在6.035~30.175 μg/mL范围内线性关系良好（R²=0.9997）；异嗪皮啶和迷迭香酸的平均回收率分别为99.13%和100.30%，RSD分别为1.98%和0.02%（n=6）。结果表明，建立的定性及定量分析方法可操作性强，结果准确可靠，可用于肿节风三清颗粒中肿节风、射干、甘草的鉴别和指标性成分异嗪皮啶和迷迭香酸的含量测定，为肿节风三清颗粒的质量标准提供了方法学依据。

关键词: 肿节风三清颗粒; 异嗪皮啶; 迷迭香酸; 质量标准; 薄层色谱; 高效液相色谱

Abstract: This study was aimed to establish a method for the identification and content determination of Zhongjiefeng Sanqing granule,so as to provide a methodological basis for the quality standards of the preparation.The Zhongjiefeng,Shegan and Gancao in Zhongjiefeng Sanqing granule were qualitatively analyzed by thin layer chromatography (TLC);The content of isofraxidin and rosmarinic acid in the preparation was determined by high performance liquid chromatography (HPLC).The results of TLC showed that Zhongjiefeng,Shegan and Gancao in Zhongjiefeng Sanqing granule had the same fluorescent spots at the same position as the corresponding standard medicinal materials or standards,while the negative samples had no characteristic fluorescent spots.The HPLC results showed that the linear relationship of isofraxidin in the range of 3.795 to 18.975 μg/mL and rosmarinic acid in the range of 6.035 to 30.175 μg/mL were both good,and the R² value were 0.9995 and 0.9997,respectively.The average recoveries of isofraxidin and rosmarinic acid were 99.13% and 100.30%,respectively;And the RSD was 1.98% and 0.02% (n=6),respectively.The results showed that the established identification and quantitative analysis methods were operability,accurate and reliable,and could be used for the identification of Zhongjiefeng,Shegan,Gancao and the determination of the components of isofraxidin and rosmarinic acid in Zhongjiefeng Sanqing granule,which was important to provide a methodological basis for establishment of the quality standard of Zhongjiefeng Sanqing granule.

Key words: Zhongjiefeng Sanqing granule; isofraxidin; rosmarinic acid; quality standard; thin layer chromatography; high performance liquid chromatography

中图分类号:

S853.7

邓锐涵, 林柏良, 杨柳, 陆彦蓉, 彭健波, 吴文健, 杨磊, 郝智慧, 杨善忠, 何家康. 肿节风三清颗粒的薄层色谱鉴别和含量测定[J]. 中国畜牧兽医, 2019, 46(10): 3095-3105.

DENG Ruihan, LIN Bailiang, YANG Liu, LU Yanrong, PENG Jianbo, WU Wenjian, YANG Lei, HAO Zhihui, YANG Shanzhong, HE Jiakang. Thin Layer Chromatography Identification and Content Determination of Zhongjiefeng Sanqing Granule[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3095-3105.

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