新型鸭呼肠孤病毒QY株σB蛋白基因特征、二级结构预测及其细胞抗原表位分析

doi:10.16431/j.cnki.1671-7236.2019.10.005

中国畜牧兽医 ›› 2019, Vol. 46 ›› Issue (10): 2851-2859.doi: 10.16431/j.cnki.1671-7236.2019.10.005

新型鸭呼肠孤病毒QY株σB蛋白基因特征、二级结构预测及其细胞抗原表位分析

吴阳开¹, 范文胜², 张雪莲³, 李智丽³, 郭锦玥³, 李文锋³, 黄淑坚³

1. 广东容大生物股份有限公司, 清远 511517;
2. 广西大学动物科学技术学院, 南宁 530005;
3. 佛山科学技术学院生命科学与工程学院, 佛山 528231

收稿日期:2019-03-28 出版日期:2019-10-20 发布日期:2019-10-21
通讯作者: 黄淑坚 E-mail:sjhuang.foshan@163.com
作者简介:吴阳开(1977-),男,江西抚州人,硕士,兽医师,研究方向:动物保健品的开发及推广,E-mail:392835434@qq.com;范文胜(1983-),男,广东清远人,博士,研究方向:禽病防治与病原分子生物学,E-mail:2003424318@163.com吴阳开与范文胜对本文具有同等贡献,并列为第一作者
基金资助:
广东省水禽产业技术体系创新团队项目；广东省教育厅预防兽医学重点实验室项目（2014KTSPT037）；佛山科学技术学院动物新发疫病防控重点实验室开放基金（KLPREAD201801-08）

Analysis for Characteristics of the New-type Duck Reovirus σB Protein and Prediction of Secondary Structure and B- and T-cell Epitopes

WU Yangkai¹, FAN Wensheng², ZHANG Xuelian³, LI Zhili³, GUO Jinyue³, LI Wenfeng³, HUANG Shujian³

1. Guangdong Rongda Biology Co., Ltd., Qingyuan 511517, China;
2. College of Animal Science and Technology, Guangxi University, Nanning 530005, China;
3. College of Life Science and Engineering, Foshan University, Foshan 528231, China

Received:2019-03-28 Online:2019-10-20 Published:2019-10-21

摘要/Abstract

摘要： 本研究旨在对新型鸭呼肠孤病毒（NDRV）QY株σB蛋白的遗传变异规律和结构及功能进行分析。从GenBank数据库中获取QY株和25株参考株的σB蛋白编码序列，通过Mega 6.0软件进行序列比对分析和系统进化树构建；使用Datamonkey软件进行选择压力分析；运用生物信息学软件预测QY株σB蛋白二级结构功能及B细胞和T细胞抗原表位。相似性分析结果显示，NDRV QY株与国内其他地区分离的鸭呼肠孤病毒（DRV）氨基酸相似性达到94.9%~98.9%，与禽呼肠孤病毒（ARV）及番鸭呼肠孤病毒（MDRV）的相似性仅为66.5%~68.4%和67.6%~68.4%；选择压力分析显示，σB蛋白承受净化选择压力，但存在一个正向选择位点；σB蛋白属于亲水蛋白，不具有信号肽和跨膜区，含有潜在的O-糖基化位点；结构预测分析显示，σB蛋白具有α-螺旋、β-折叠、β-转角及无规则卷曲等丰富的二级结构；表位分析显示，σB蛋白含有潜在的B细胞和T细胞抗原表位。本研究成功进行了QY株σB蛋白的基因特征和结构功能预测及其细胞表位分析，为深入了解该蛋白的免疫学特性及研发NDRV新型疫苗奠定了基础。

关键词: 新型鸭呼肠孤病毒(NDRV); σB蛋白; 基因特征; 二级结构; 抗原表位

Abstract: The purpose of this study was to analyze the genetic variation,structure and function of σB protein of a new duck reovirus (NDRV) QY strain.The coding sequences of σB protein of QY strain and 25 reference strains were obtained from GenBank database.Sequence alignment analysis and phylogenetic tree construction were carried out by Mega 6.0 software.Selective pressure analysis was carried out by Datamonkey software.Secondary structure and function of σB protein of QY strain and antigen epitopes of B and T cells were predicted by bioinformatics software.Similarity analysis showed that the amino acid similarity of NDRV QY strain with duck reovirus (DRV) isolated from other regions in China reached 94.9% to 98.9%,and the similarity with avian reovirus (ARV) and Muscovy duck reovirus (MDRV) were only 66.5% to 68.4% and 67.6% to 68.4%,respectively.Selective pressure analysis showed that σB protein was subjected to purification selective pressure,but one positively selected site was detected.The σB protein belonged to hydrophilic protein,had no signal peptide and transmembrane region,and contained potential O-glycosylation sites.Structural prediction analysis showed that σB protein had abundant secondary structures such as alpha helix,beta fold,beta turn and random coil.Epitope analysis showed that σB protein contained potential B- and T-cell epitopes.This study successfully predicted the gene characteristics,structure and function of σB protein of QY strain and analyzed its cell epitope,which laid a foundation for further understanding the immunological characteristics of the protein and developing a new NDRV vaccine.

Key words: new-type duck reovirus (NDRV); σB protein; genetic characteristics; secondary structure; epitope

中图分类号:

S852.65

吴阳开, 范文胜, 张雪莲, 李智丽, 郭锦玥, 李文锋, 黄淑坚. 新型鸭呼肠孤病毒QY株σB蛋白基因特征、二级结构预测及其细胞抗原表位分析[J]. 中国畜牧兽医, 2019, 46(10): 2851-2859.

WU Yangkai, FAN Wensheng, ZHANG Xuelian, LI Zhili, GUO Jinyue, LI Wenfeng, HUANG Shujian. Analysis for Characteristics of the New-type Duck Reovirus σB Protein and Prediction of Secondary Structure and B- and T-cell Epitopes[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 2851-2859.

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新型鸭呼肠孤病毒QY株σB蛋白基因特征、二级结构预测及其细胞抗原表位分析

Analysis for Characteristics of the New-type Duck Reovirus σB Protein and Prediction of Secondary Structure and B- and T-cell Epitopes

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