氟苯尼考纳米乳制备及其抑菌效果观察

doi:10.16431/j.cnki.1671-7236.2018.10.032

摘要/Abstract

摘要：

为制备氟苯尼考纳米乳并了解其抑菌效果，本试验进行了处方筛选、物理性质考察、抑菌圈试验，通过测定氟苯尼考在多种油相中的溶解度，确定最佳油相；再以乳化剂-助乳化剂（Smix）体积比Kv值及Smix-油相体积比为考察指标，结合伪三元相图筛选最佳处方组成；利用染色法鉴别纳米乳类型，通过透射电镜观察纳米乳微观形态；通过激光粒度分析仪测定粒度分布、Zeta电位；采用离心及长期试验考察其稳定性；经抑菌圈试验观察氟苯尼考纳米乳对金黄色葡萄球菌、大肠埃希菌、微杆菌、枯草芽孢杆菌的体外抑菌效果。结果显示，橄榄油为最佳油相，EL-40为最佳乳化剂，丙三醇为最佳助乳化剂，优选的Smix体积比Kv值为2.0，Smix-油相体积比为8：2。氟苯尼考纳米乳最佳处方组成为：氟苯尼考120 mg，N，N-二甲基甲酰胺0.1 mL，橄榄油2 mL，EL-40 5.7 mL，丙三醇2.3 mL，水6 mL。氟苯尼考纳米乳为水包油（O/W）型，外观呈球形，大小均匀无黏连，平均粒径28 nm，粒径呈正态分布，Zeta电位为-0.454 mV，经离心试验及长期试验样品稳定，对4种常见菌体外抑菌效果强于同浓度氟苯尼考溶液。本试验结果表明，氟苯尼考纳米乳制备方法简单、可行，且纳米乳稳定，抑菌效果良好，在畜牧养殖中具有潜在应用价值。

关键词: 氟苯尼考; 纳米乳; 伪三元相图; 抑菌效果

Abstract:

In order to successfully prepare florfenicol nanoemulsion and investigate its bacteriostatic effect,the prescription screening,physical properties investigation and bacteriostatic activity test were conducted.The oil phase,emulsifier and coemulsifier were optimized by determining the solubility of florfenicol in oil phases,emulsifiers and coemulsifier,respectively.The optimum formula was designed based on Kv of emulsifier-coemulsifier (Smix) and Smix-oil phase volume ratio which was selected using pseudo-ternary phase diagrm.Florfenicol nanoemulsion was distinguished by staining method,and the configuration of florfenicol nanoemulsion were measured by TEM,the particle size and Zeta potential were measured by laser particle size analyzer.The stability was investigated by a centrifugation and a long-term test.The antibacterial effect of florfenicol nanoemulsion on Staphylococcus aureus,Escherichia coli,Microbacterium and Bacillus subtilis was studied by a bacteriostatic activity test.The results showed that olive oil was the optimized oil phase,EL-40 was the optimized emulsifier,glycerin was the optimized coemulsifier,the optimized ratio of Kv was 2.0,and the volume ratio of Smix-oil was 8:2.The optimum formula of florfenicol nanoemulsion was the composition of fluorobenicol (120 mg),N,N-dimethylformamide(0.1 mL),olive oil (2 mL),EL-40(5.7 mL),propanol(2.3 mL),and distilled water(6 mL).The uniform size and no adhesion droplet shape of florfenicol nanoemulsion was O/W,with the average diameter of 28 nm and Zeta potential of -0.454 mV,maintained to be stable up to six months.The florfenicol nanoemulsion performanced a better antibacterial effect than the same concentration of fluorphenicol solution for four bacterias.The results demonstrated that the preparation method of florfenicol nanoemulsion was simple,feasible,and showed high stability and good bacteriostasis.The study proved that the florfenicol nanoemulsion had potential application value in the animal husbandry and aquaculture.

Key words: florfenicol; nano emulsion; pseudoternary phase diagrm; antibacterial effects

中图分类号:

S859.79

时菲菲, 秦枫, 王秀君, 马琪伦, 顾疏桐, 魏亚兰, 李杨. 氟苯尼考纳米乳制备及其抑菌效果观察[J]. 《中国畜牧兽医》, 2018, 45(10): 2936-2942.

SHI Feifei, QIN Feng, WANG Xiujun, MA Qilun, GU Shutong, WEI Yalan, LI Yang. Preparation of Florfenicol Nanoemulsion and Observation of Its Antibacterial Effect[J]. , 2018, 45(10): 2936-2942.

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