水貂志贺氏菌分离鉴定及药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2017.11.038

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (11): 3379-3384.doi: 10.16431/j.cnki.1671-7236.2017.11.038

水貂志贺氏菌分离鉴定及药物敏感性分析

孙娜¹, 陈强², 杨艳玲¹, 郭利¹, 冯秋菊³, 孙铭⁴, 程世鹏¹

1. 中国农业科学院特产研究所, 农业部经济动物疫病重点实验室, 长春 130112;
2. 吉林特研生物技术有限责任公司, 长春 130112;
3. 四川省凉山州木里县农牧局, 木里 615899;
4. 吉林省通化市动物疫病预防控制中心, 通化 134000

收稿日期:2017-05-12 出版日期:2017-11-20 发布日期:2017-11-21
通讯作者: 程世鹏 E-mail:tcscsp@126.com
作者简介:孙娜(1983-),女,吉林辉南人,博士,副研究员,研究方向:细菌耐药性,E-mail:sunna@caas.cn;陈强(1982-),男,吉林敦化人,硕士,研究方向:毛皮动物诊断,E-mail:chenqiang821121@126.com
基金资助:
吉林省科技发展计划项目（20150520128JH）；中国农业科学院创新工程基金（20140204066NY）；公益性行业（农业）科研专项经费项目任务书（201303042）

Isolation, Identification and Drug Sensitivity Analysis of Shigella from Minks

SUN Na¹, CHEN Qiang², YANG Yan-ling¹, GUO Li¹, FENG Qiu-ju³, SUN Ming⁴, CHENG Shi-peng¹

1. Key Laboratory of Special Animal Epidemic Disease Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
2. JL TEYAN Biological Technology Limited Liability Company, Changchun 130112, China;
3. Muli County Agriculture and Animal Husbandry Bureau of Liangshan Prefecture in Sichuan Province, Muli 615899, China;
4. Animal Epidemic Disease Prevention and Control Center of Tonghua City in Jilin Province, Tonghua 134000, China

Received:2017-05-12 Online:2017-11-20 Published:2017-11-21

摘要/Abstract

摘要：

试验旨在对吉林省某水貂养殖场送检的3只具有典型腹泻症状的病死水貂的小肠和肠内容物样品进行细菌分离鉴定及耐药情况分析，为临床治疗提供参考。通过细菌分离纯化和PCR方法对分离菌株进行鉴定，对BALB/c小鼠进行菌液注射来检测菌株的致病性。采用K-B药敏法检测菌株对常用药物的敏感性，并通过PCR方法检测其耐药基因和Ⅰ类整合子的携带情况。结果显示，分离得到3株志贺氏菌，致病性检测试验显示分离菌可引起小鼠腹泻。药物敏感性试验结果显示，3株志贺氏菌对氟喹诺酮类药物、头孢类药物、氟苯尼考和多黏菌素较敏感，对氨基糖苷类、四环素、氯霉素、青霉素、氨苄西林耐药。耐药基因检测结果显示，3株志贺氏菌共检测出7种耐药基因bla_TEM-1、aadA1、aac（3'）-Ⅱc、aac（6'）-Ⅰb、aph（3'）-Ⅶ、tet（M）、cat2及携带aadA1基因盒的Ⅰ类整合子。结果表明，分离的3株志贺氏菌均具有致病性，可引起小鼠腹泻，主要表现为多重耐药现象，携带的耐药基因呈多样性，为临床治疗带来巨大影响。

关键词: 水貂; 志贺氏菌; 腹泻; 药物敏感性

Abstract:

In order to provide therapeutical guidance for drug admistration, the bacteria of three sick minks suffering from typical diarrhea symptoms provided by mink farms in Jilin province were isolated and identified, and the drug sensitivity was tested. The bacteria were isolated with TSA plates, and identified using biochemical methods and PCR assay. The virulence of the isolates was determined by infecting BALB/c mice. The antimicrobial susceptibility of the isolates to antimicrobial agents was investigated using the K-B method. PCR was used to detect the resistance genes and Ⅰ integrons. A total of 3 Shigella isolates were obtained from sick minks. The virulent determination showed that all isolates could cause mice diarrhea. The drug sensitivity results showed that 3 strains were sensitive to fluoroquinolone, cephalosporin, florfenicol and polymyxin, but they were resistant to aminoglycoside, tetracycline, chloramphenicol, penicillin and ampicillin. There were seven resistance genes were detected,bla_TEM-1,aadA1, aac(3')-Ⅱc, aac(6')-Ⅰb, aph(3')-Ⅶ, tet(M), cat2 and three class Ⅰ integrons carrying aadA 1 gene cassette. All of the isolates were virulent and caused the mice diarrhea. The resistance of the 3 strains were very serious and mainly for multiple drug resistance phenomenon. The resistance genes detected in the mink were various, and could bring enormous implications for clinical treatment.

Key words: mink; Shigella; diarrhea; drug sensitivity

中图分类号:

S858.92

孙娜, 陈强, 杨艳玲, 郭利, 冯秋菊, 孙铭, 程世鹏. 水貂志贺氏菌分离鉴定及药物敏感性分析[J]. 《中国畜牧兽医》, 2017, 44(11): 3379-3384.

SUN Na, CHEN Qiang, YANG Yan-ling, GUO Li, FENG Qiu-ju, SUN Ming, CHENG Shi-peng. Isolation, Identification and Drug Sensitivity Analysis of Shigella from Minks[J]. , 2017, 44(11): 3379-3384.

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水貂志贺氏菌分离鉴定及药物敏感性分析

Isolation, Identification and Drug Sensitivity Analysis of Shigella from Minks

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