鸡血细胞药物代谢模型的建立与应用

doi:10.16431/j.cnki.1671-7236.2017.10.034

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (10): 3070-3076.doi: 10.16431/j.cnki.1671-7236.2017.10.034

鸡血细胞药物代谢模型的建立与应用

张小庆, 汤晓艳, 杨小体, 沈习习

中国农业科学院农业质量标准与检测技术研究所, 农业部农产品质量安全重点实验室, 北京 100081

收稿日期:2017-04-26 出版日期:2017-10-20 发布日期:2017-10-20
通讯作者: 汤晓艳 E-mail:txycaas@126.com
作者简介:张小庆(1991-),女,河北保定人,硕士,研究方向:食品质量与安全,E-mail:915778355@qq.com
基金资助:
中国农业科学院科技创新工程项目

The Establishment and Application of Metabolic Model of Blood Cells in Chicken

ZHANG Xiao-qing, TANG Xiao-yan, YANG Xiao-ti, SHEN Xi-xi

Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2017-04-26 Online:2017-10-20 Published:2017-10-20

摘要/Abstract

摘要：

试验旨在建立鸡血细胞药物代谢模型，并用利巴韦林进行验证。本试验比较了3种不同培养基（L-15、M199和RPMI1640培养基）及添加不同浓度胎牛血清的细胞培养效果，采用MTT法测细胞活力，确定最佳给药时间，之后用利巴韦林进行给药，检测培养液中利巴韦林及其代谢物（TCONH₂和RTCOOH）含量。结果发现，3种培养基中用L-15培养基培养时细胞存活率最高，胎牛血清添加浓度为10%时血细胞状态较好；血细胞活力检测表明其最佳给药时间为培养3 h；利巴韦林给药后，其含量随着时间的延长而降低，TCONH₂和RTCOOH在给药0.5 h时迅速产生，给药3 h后其浓度变化趋于平缓。综上所述，本试验建立的鸡血细胞代谢模型操作简便，用添加10%胎牛血清的L-15培养基培养效果较好，利巴韦林给药试验表明，鸡血细胞存在一定的代谢转化功能，该鸡血细胞代谢模型可用于某些药物的体外代谢研究。

关键词: 鸡血细胞; 利巴韦林; 药物体外代谢

Abstract:

The experiment was aimed to establish a drug metabolic model of the blood cells in chicken. The effects of three kinds of culture medium (L-15,M199 and RPMI1640) and different concentrations of fetal bovine serum (FBS) were compared to optimize the culture condition of blood cells,and the proper time of medication was determined after the cell vitality was measured by MTT assay. The content of ribavirin and its metabolites (TCONH₂ and RTCOOH) were detected after the ribavirin was dosed. The results showed that the cell viability was highest when the blood cells cultured in L-15 medium,the state of blood cells was better when 10% FBS was added to the medium. The best time for medication was when blood cells were cultured for 3 h. The content of ribavirin was decreased with the time of administration,the metabolites of ribavirin were increased quickly after half an hour, it changed slowly after 3 h. In conclusion,the metabolic model of blood cells in chicken was successfully established,and the blood cells cultured in vitro were better using L-15 medium supplemented with 10% FBS. The metabolic transformation function of blood cells in chicken was indicated by the medication test of ribavirin and it could be used to study the drug metabolism in vitro.

Key words: chicken blood cells; ribavirin; drug metabolism in vitro

中图分类号:

R969.1

张小庆, 汤晓艳, 杨小体, 沈习习. 鸡血细胞药物代谢模型的建立与应用[J]. 《中国畜牧兽医》, 2017, 44(10): 3070-3076.

ZHANG Xiao-qing, TANG Xiao-yan, YANG Xiao-ti, SHEN Xi-xi. The Establishment and Application of Metabolic Model of Blood Cells in Chicken[J]. , 2017, 44(10): 3070-3076.

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鸡血细胞药物代谢模型的建立与应用

The Establishment and Application of Metabolic Model of Blood Cells in Chicken

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