一种新型仔猪先天性震颤及其病原的初步研究

doi:10.16431/j.cnki.1671-7236.2017.07.034

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (7): 2147-2154.doi: 10.16431/j.cnki.1671-7236.2017.07.034

一种新型仔猪先天性震颤及其病原的初步研究

张文波^1,2, 吴松松¹, 邓舜洲¹, 周荷田², 张志庆², 杨丹凤², 曹亮亮²

1. 江西农业大学动物科学技术学院, 南昌 330045;
2. 江西金伊博生物科技有限公司, 南昌 330013

收稿日期:2017-01-17 出版日期:2017-07-20 发布日期:2017-07-22
通讯作者: 邓舜洲 E-mail:shzhdeng@163.com
作者简介:张文波(1972-),男,河南三门峡人,博士,研究方向:动物传染病病原及防控,E-mail:hnzwb22@163.com
基金资助:
江西省科技支撑计划项目（20121BBF60031）；江西省现代农业产业技术体系建设专项资金（JXARS-03）

Preliminary Study on a Novel Atypical Congenital Tremor of Piglets and Its Etiology

ZHANG Wen-bo^1,2, WU Song-song¹, DENG Shun-zhou¹, ZHOU He-tian², ZHANG Zhi-qing², YANG Dan-feng², CAO Liang-liang²

1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
2. Jiangxi JIN YI BO Biological Technology Co., Ltd., Nanchang 330013, China

Received:2017-01-17 Online:2017-07-20 Published:2017-07-22

摘要/Abstract

摘要：

为了解国内猪群是否存在新型仔猪先天性震颤及其病原，本试验对临床疑似仔猪先天性震颤病例进行剖检，观察组织脏器的病理变化；根据GenBank上登录的瘟病毒基因组序列设计2对引物，以典型震颤仔猪的病料RNA为模板，进行RT-PCR，并对阳性产物进行测序；用DNAStar和Mega 7.0软件对所测序列进行分析，绘制系统进化树。结果表明，疑似仔猪先天性震颤病猪的组织脏器病理变化与猪瘟的病理变化相似，但无明显的脾脏梗死和淋巴结周边出血；从采集的病料中均扩增到与预期大小基本一致的条带；所测序列经BLAST比对，均为仔猪先天性震颤瘟病毒（APPV）的相应基因序列片段；系统进化树结果显示，哺乳动物瘟病毒可分为两大进化分支，APPV为单独一分支，其他瘟病毒为另一分支，两分支的同源性低于70%；所测的NS2-3和NS5B基因序列与GenBank上登录的APPV相应序列同源性在76.9%~98.8%之间，表明本研究所测定的序列是APPV的基因组序列；测定的7条NS2-3基因序列之间的同源性在89.7%~94.8%之间，12条NS5B基因序列的同源性在82.2%~100.0%之间。本试验首次证实国内猪群中存在新型仔猪先天性震颤病及其病原（APPV）。

关键词: 仔猪先天性震颤; 瘟病毒; 仔猪先天性震颤瘟病毒(APPV); 序列分析

Abstract:

To investigate the infection of atypical porcine pestivirus (APPV) in China, autopsywas carried out for clinical suspected piglet of congenital tremor, viscera pathologic changes were observed. Two pairs of primersfor detection of the NS2-3 and NS5B genes by RT-PCR were designed according to the genome of APPV. The positive product was sequenced using DNAStar and Mega 7.0 softwares,and drawing the molecular evolution trees. The results showed that the lesions were similar to classical swine fever, but without infarction of the spleen and peripheral hemorrhage of the mandibular lymph node.Epidemic materials amplified products were consistent with the expected size, the sequences were APPV fragments by BLAST. APPV and other pestivirus belonged to two branch with the homologies about 70%. The homologies of NS2-3 and NS5B genes among the isolates and the APPV reference strains were about 76.9% to 98.8%, and the homologies of NS2-3 gene of 7 isolates were 89.7% to 94.8%,the homologies of NS5B gene of 12 isolates were 82.2% to 100.0%. This was the first report for the infection of APPV in piglets in China.

Key words: congenital tremor of piglets; pestivirus; atypical porcine pestivirus (APPV); sequence analysis

中图分类号:

S852.65

张文波, 吴松松, 邓舜洲, 周荷田, 张志庆, 杨丹凤, 曹亮亮. 一种新型仔猪先天性震颤及其病原的初步研究[J]. 《中国畜牧兽医》, 2017, 44(7): 2147-2154.

ZHANG Wen-bo, WU Song-song, DENG Shun-zhou, ZHOU He-tian, ZHANG Zhi-qing, YANG Dan-feng, CAO Liang-liang. Preliminary Study on a Novel Atypical Congenital Tremor of Piglets and Its Etiology[J]. , 2017, 44(7): 2147-2154.

参考文献

[1] 宣长和,马春全,陈志宝,等.猪病学[M].第3版.北京:中国农业大学出版社,2010.
[2] Blomstr m A L, Ley C, Jacobson M. Astrovirus as a possible cause of congenital tremor type AⅡ in piglets[J].Acta Vet Scand, 2014, 56(1):1-6.
[3] Patterson D S, Sweasey D, Brush P J. Neurochemistry of the spinal cord in British Saddleback piglets affected with congenital tremor, type A-Ⅳ, a second form of hereditary cerebrospinal hypomyelinogenesis[J].J Neurochem,1973,21(2):397-406.
[4] Baumgartner B G, Brenig B. The role of proteolipid proteins in the development of congenital tremors type AⅢ:A review[J]. Dtsch Tierarztl Wochenschr, 1996,103(10):404-407.
[5] Done J T, Woolley J, Upcott D H, et al. Porcine congenital tremor type AⅠ:Spinal cord morphometry[J]. Zentralbl Veterinarmed A, 1984, 31(2):81-90.
[6] Done J T, Woolley J, Upcott D H, et al. Porcine congenital tremor type AⅡ:Spinal cord morphometry[J]. Br Vet J,1986, 142(2):145-150.
[7] Harding J D, Done J T, Harbourne J F,et al. Congenital tremor type A 3 in pigs:An hereditary sex-linked cerebrospinal hypomyelinogenesis[J]. Vet Rec, 1973,92(20):527-529.
[8] Knox B, Askaa J, Basse A,et al. Congenital ataxia and tremor with cerebellar hypoplasia in piglets borne by sows treated with Neguvon vet. (metrifonate, trichlorfon) during pregnancy[J]. Nord Vet Med, 1978,30(12):538-545.
[9] Vannier P, Plateau E, Tillon J P.Congenital tremor in pigs farrowed from sows given hog cholera virus during pregnancy[J].Am J Vet Res,1981,42(1):135-137.
[10] Vilcek S, Belák S. Organization and diversity of the 3'-noncoding region of classical swine fever virus genome[J].Virus Genes,1997,15(2):181-186.
[11] Ha Y, Jung K, Chae C.Lack of evidence of porcine circovirus type 1 and type 2 infection in piglets with congenital tremors in Korea[J].Vet Rec, 2005,156(12):383-384.
[12] 陈燕珊,邓素军,陈俊伟,等.AⅡ型仔猪先天性震颤病的防控研究进展[J].广东畜牧兽医科技,2011,36(1):11-12.
[13] Kennedy S, Segalés J, Rovira A,et al. Absence of evidence of porcine circovirus infection in piglets with congenital tremors[J]. J Vet Diagn Invest,2003,15(2):151-156.
[14] Hause B M, Collin E A, Peddireddi L, et al. Discovery of a novel putative atypical porcine pestivirus in pigs in the USA[J]. J Gen Virol, 2015, 96(10):2994-2998.
[15] Beer M, Wernike K, Dräger C, et al. High prevalence of highly variable atypical porcine pestiviruses found in Germany[J].Transbound Emerg Dis, 2016,Epub ahead of print.
[16] 戴爱玲.仔猪先天性震颤的流行病学调查及病原学初步研究[D].福州:福建农林大学,2011.
[17] Arruda B L, Arruda P H, Magstadt D R,et al. Identification of a divergent lineage porcine pestivirus in nursing piglets with congenital tremors and reproduction of disease following experimental inoculation[J]. PLoS One, 2016,11(2):e0150104.
[18] de Groof A, Deijs M, Guelen L, et al.Atypical porcine pestivirus:A possible cause of congenital tremor type A-Ⅱ in newborn piglet[J]. Viruses,2016,8(10):271.
[19] Postel A, Hansmann F, Baechlein C,et al. Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremor. Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremor[J]. Sci Rep, 2016, 6:27735.
[20] Schwarz L, Riedel C, H gler S,et al. Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence[J]. Vet Res,2017, 48:1.

一种新型仔猪先天性震颤及其病原的初步研究

Preliminary Study on a Novel Atypical Congenital Tremor of Piglets and Its Etiology

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张芳源, 蔺雅婷, 杨大为, 陈虎, 李桂梅, 单虎. 非洲猪瘟病毒P30蛋白原核表达及间接ELISA检测方法建立[J]. 中国畜牧兽医, 2023, 50(5): 2012-2022.
[2]	侯浩宇, 郑南南, 吴宏举, 陈寅龙, 李潮, 张昂克, 姬鹏超, 万博, 杜永坤, 张改平. 非洲猪瘟病毒p30蛋白单克隆抗体制备和鉴定[J]. 中国畜牧兽医, 2023, 50(3): 1241-1249.
[3]	李朋霏, 宋晓明, 周保琨, 曹志, 张洪亮, 马清霞, 单虎. 用于非洲猪瘟病毒核酸检测的假病毒阳性对照品的制备和标定[J]. 中国畜牧兽医, 2023, 50(2): 647-655.
[4]	张凯, 张春平, 戈胜强, 孙春喜, 程杰, 伏春雨, 王刚, 牛星, 彭军. 非洲猪瘟病毒p22蛋白表达、多克隆抗体制备及间接ELISA方法的建立[J]. 中国畜牧兽医, 2023, 50(1): 270-279.
[5]	张梦杨, 何健, 刘阳坤, 姚伦广. 非洲猪瘟病毒p37蛋白生物信息学分析及其多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(1): 300-309.
[6]	徐娅玲, 张霁辉, 牛熙, 李升, 黄世会, 冉雪琴, 王嘉福. 非洲猪瘟病毒P72蛋白合成肽疫苗的构建及其免疫效力评估[J]. 中国畜牧兽医, 2022, 49(8): 3083-3090.
[7]	陈世钰, 崔帅, 蒋亚君, 鑫婷, 王洋, 郝玉欣, 黄建新, 郭晓宇, 朱鸿飞, 吴佳俊, 贾红. 非洲猪瘟病毒感染后猪外周血淋巴细胞Toll样受体信号通路的lncRNA-mRNA分析[J]. 中国畜牧兽医, 2022, 49(7): 2677-2687.
[8]	张文燕, 王亚文, 袁晨, 冯亚文, 滕召剑, 商佳亮, 逯纪成, 宋勤叶. 基于非洲猪瘟病毒截短p72蛋白的间接ELISA抗体检测方法的建立[J]. 中国畜牧兽医, 2022, 49(7): 2688-2697.
[9]	宋春, 王柏林, 李梅, 文明, 王开功, 陈常秀, 程振涛. 鸡滑液囊支原体贵州株的分离鉴定及VlhA基因序列分析[J]. 中国畜牧兽医, 2022, 49(4): 1516-1523.
[10]	韦云芳, 李飞翔, 星云, 黄庆国, 李居东, 万九生, 陈方良. 昆明犬IGF2基因克隆、生物信息学分析及其时空表达研究[J]. 中国畜牧兽医, 2022, 49(3): 866-875.
[11]	何献铭, 任广彩, 叶俊贤, 兰虹, 刘郁夫, 熊挺, 杨泽坤, 徐婷, 陈瑞爱. 1株传染性法氏囊病病毒的分离鉴定及VP2基因序列分析[J]. 中国畜牧兽医, 2022, 49(3): 1005-1014.
[12]	蔡杰, 吴佳怡, 潘苑霞, 呼高伟, 张林州. 1株大黄鱼源金黄杆菌的分离鉴定和耐药性分析[J]. 中国畜牧兽医, 2022, 49(3): 1135-1143.
[13]	胡晓冰, 林标声, 王振伟, 郑黎静. 1株利用乳酸的猪源丁酸梭菌的分离鉴定及基因组序列分析[J]. 中国畜牧兽医, 2022, 49(2): 569-578.
[14]	张艳敏, 周亚南, 曹磊, 田园, 刘旭平, 谭文松, 赵亮. 猪瘟病毒E2-GM-CSF融合蛋白在HEK293T细胞中的表达和免疫原性分析[J]. 中国畜牧兽医, 2022, 49(2): 669-676.
[15]	许春梅, 邵明珠, 王心悦, 林佳迪, 郭建雄, 张祥胤, 童德文, 梁瑞英, 赵晓民. 非洲猪瘟病毒D250R蛋白生物信息学分析及多克隆抗体制备[J]. 中国畜牧兽医, 2022, 49(12): 4745-4755.