养殖锦鲤鲤鱼浮肿病的检测与鉴定

doi:10.16431/j.cnki.1671-7236.2017.02.043

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (2): 613-618.doi: 10.16431/j.cnki.1671-7236.2017.02.043

• 临床兽医 • 上一篇

养殖锦鲤鲤鱼浮肿病的检测与鉴定

徐立蒲¹, 王小亮¹, 张文¹, 潘勇¹, 景宏丽², 王静波¹, 王姝¹, 曹欢¹, 王澎¹, 那立海¹, 江育林²

1. 北京市水产技术推广站, 北京 100021;
2. 中国检验检疫科学研究院动物检疫研究所, 北京 100029

收稿日期:2016-06-22 出版日期:2017-02-20 发布日期:2017-02-25
通讯作者: 徐立蒲 E-mail:xulipu001@163.com
作者简介:徐立蒲(1972-), 男, 黑龙江哈尔滨人, 博士, 研究员, 研究方向:鱼病诊断防控
基金资助:
农业部动物疫情监测与防治项目；北京市农业试验示范项目（20160117）；现代农业产业技术体系北京市观赏鱼创新团队（GSY-02-09）

Testing and Identification of Carp Edema Virus Disease in Cultured Koi

XU Li-pu¹, WANG Xiao-liang¹, ZHANG Wen¹, PAN Yong¹, JING Hong-li², WANG Jing-bo¹, WANG Shu¹, CAO Huan¹, WANG Peng¹, NA Li-hai¹, JIANG Yu-lin²

1. Beijing Aquaculture Technology Extention Station, Beijing 100021, China;
2. Institute of Animal Quatantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China

Received:2016-06-22 Online:2017-02-20 Published:2017-02-25

摘要/Abstract

摘要：

北京房山某锦鲤养殖场锦鲤发生大量死亡，患病锦鲤呈烂鳃、肾脏糜烂、身体浮肿；症状类似锦鲤疱疹病毒（koi herpes virus，KHV）感染，但经PCR检测排除了KHV感染。为进一步确定病原，对发病鱼进行了临床症状观察、病毒分离、细菌分离培养、病鱼组织超薄切片电镜观察和PCR扩增等检测。通过病鱼组织超薄切片电镜观察，在肾脏内可见200 nm×400 nm的痘病毒样颗粒。抽提病毒核酸后，用已知的鲤鱼浮肿病毒（carp edema virus，CEV）的保守序列设计2对引物进行PCR扩增，扩增出548和180 bp片段，测序结果和GenBank公布的CEV H504株序列完全一致。根据发病鱼临床症状和实验室检测结果，最终确定该病为CEV引起的鲤鱼浮肿病。这是在中国首次发现的鲤鱼浮肿病。

关键词: 鲤鱼浮肿病毒; 鲤鱼浮肿病; 锦鲤疱疹病毒; 锦鲤

Abstract:

An acute infectious diseases occurred in a koi farm in Fangshan district, Beijing, and it resulted in mortalities of more than 50%.The main symptoms of sick koi were gills necrosis, kidney erosion and edema,which were similar to the clinical signs of koi herpes virus disease (KHVD).But PCR tests showed negative results for KHV. For further diagnosis, bacterial cultures, transmission electron microscopy studies, virus isolation and PCR tests were used. Electron microscopic observation revealed pox virus particles having a size of about 200 nm×400 nm in the kidney. 548 and 180 bp fragments were amplified from organs of sick koi by PCR method using specific primers of carp edema virus (CEV). The fragments were sequenced and analysed. The results showed that they were shared 100% nucleotide identity with CEV-H504. All the results indicated that this disease was carp edema virus disease, caused by a kind of pox virus, CEV. This was the first report on the CEV of cultured koi in China.

Key words: carp edema virus; carp edema virus disease; koi herpes virus; koi

中图分类号:

S941.41

徐立蒲, 王小亮, 张文, 潘勇, 景宏丽, 王静波, 王姝, 曹欢, 王澎, 那立海, 江育林. 养殖锦鲤鲤鱼浮肿病的检测与鉴定[J]. 《中国畜牧兽医》, 2017, 44(2): 613-618.

XU Li-pu, WANG Xiao-liang, ZHANG Wen, PAN Yong, JING Hong-li, WANG Jing-bo, WANG Shu, CAO Huan, WANG Peng, NA Li-hai, JIANG Yu-lin. Testing and Identification of Carp Edema Virus Disease in Cultured Koi[J]. , 2017, 44(2): 613-618.

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养殖锦鲤鲤鱼浮肿病的检测与鉴定

Testing and Identification of Carp Edema Virus Disease in Cultured Koi

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