黑丝羽乌骨鸡精液DMA颗粒冷冻技术研究

doi:10.16431/j.cnki.1671-7236.2017.02.021

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (2): 447-455.doi: 10.16431/j.cnki.1671-7236.2017.02.021

黑丝羽乌骨鸡精液DMA颗粒冷冻技术研究

黄琳^1,2, 孙鏖², 朱立军², 彭运潮³, 王坤^1,2, 贾杏林¹, 燕海峰^1,2

1. 湖南农业大学动物医学院, 长沙 410125;
2. 湖南省畜牧兽医研究所, 长沙 410131;
3. 湖南生物机电职业技术学院, 长沙 410127

收稿日期:2016-05-13 出版日期:2017-02-20 发布日期:2017-02-25
通讯作者: 贾杏林, 燕海峰 E-mail:1376161391@qq.com;hfyan2005@163.com
作者简介:黄琳(1988-), 女, 湖南邵阳人, 硕士生, 研究方向:兽医生物技术应用, E-mail:365082026@qq.com
基金资助:
国家国际科技合作专项（2013DFG31810）；湖南省科技计划（2014WK2008）；国家水禽产业技术体系建设专项（CARS-43-39）；湖南省高等学校创新能力提升计划项目

Study on Technology of Black Silkies Chicken Semen Freezing by DMA Pellet

HUANG Lin^1,2, SUN Ao², ZHU Li-jun², PENG Yun-chao³, WANG Kun^1,2, JIA Xing-lin¹, YAN Hai-feng^1,2

1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410125, China;
2. Hunan Institute of Animal and Veterinary Science, Changsha 410131, China;
3. Hunan Biological and Elechomechanical Polytechnic, Changsha 410127, China

Received:2016-05-13 Online:2017-02-20 Published:2017-02-25

摘要/Abstract

摘要：

为研究不同稀释液与解冻装置对黑丝乌骨鸡N，N-二甲基甲酰胺（N，N-dimethylformamide，DMA）颗粒冻精解冻后精子质量的影响，试验首先采用不同稀释液对精液进行稀释并比较其解冻后精子活力与人工输精的受精率；其次，选取不同解冻管及冻精颗粒数进行恒温水浴解冻，比较其活力；最后，依据解冻后的精子活力，筛选恒温水浴、恒温漏斗、恒温板3种解冻装置各自的最佳解冻温度，并利用各自最佳解冻温度解冻后的精液进行人工输精，检测受精率。结果显示：①不同稀释液组的精子活力与受精率高低趋势一致，为LR > F > B > L组，且各组之间差异显著（P < 0.05）。②在60℃恒温水浴中，用薄壁大玻璃管解冻的精子活力最好。③不同解冻装置有各自最佳解冻活力的温度范围，恒温水浴为50~60℃（0.51~0.59）、恒温漏斗为40~45℃（0.42~0.46）、恒温板为50~55℃（0.61~0.63），每种装置最佳温度段内的精子活力差异不显著（P > 0.05）。④3种解冻装置最佳解冻状态相比：在精子活力上，60℃恒温水浴与55℃恒温板分别显著高于40℃恒温漏斗（P < 0.05），但60℃恒温水浴与55℃恒温板之间差异不显著（P > 0.05）；在受精率上，55℃恒温板最高（26.91%），60℃恒温水浴次之（23.08%）、40℃恒温漏斗最低（20.93%），三者之间差异不显著（P > 0.05）。因此，黑丝羽乌骨鸡精液应采用LR稀释液、DMA冷冻保护剂及颗粒冷冻技术，在54.9℃恒温板解冻可获得较高的受精率。

关键词: 黑丝羽乌骨鸡; 稀释液; DMA; 颗粒冻精; 解冻装置

Abstract:

The aim was to explore the effects of different kinds of dilution and thawing devices on the N,N-dimethylformamide (DMA) pellet frozen semen of Black Silkies. Firstly,the motility and fertility of the frozen semen thawed by different dilutions were compared;Then,the motility of the frozen semen was compared when the pellets were thawed using different tube and different number.Finally,the motility and fertility of the sperm thawed by three kinds of thawing devices (thermostat water bath,hotfunnel and hotplate ) were tested. The results showed that:①There was similar order of the sperm motility and fertility in the different dilution groups (LR > F > B > L),and there was significant difference among those groups (P < 0.05).②The motility was the best when the frozen semen was thawed with large thin-wall glass tube at 60℃.③The best temperature range of the 3 devices was different. The highest motility for thermostat water bath was 50 to 60℃ (0.51 to 0.59),and thermostat hotfunnel was 40 to 45℃ (0.42 to 0.46),while thermostat hotplate was 50 to 55℃ (0.61 to 0.63).There was no significant difference of the motility in the optimum temperature range for each device (P > 0.05).④The fertility of the different devices in their best thawing temperature was 26.91% (55℃,thermostat hotplate),23.08% (60℃, thermostat water bath), 20.93% (40℃, thermostat funnel),respectively,and there was no significant differences among those groups (P > 0.05).Therefore,the efficiency thawing condition for the Black Silkies frozen semen was the LR diluent,DMA cryoprotectant,pellet freezing,thawed in the thermostat hotplate at 54.9℃.

Key words: Black Silkies chicken; dilutent; DMA; pellets frozen semen; thawing device

中图分类号:

S831.2

黄琳, 孙鏖, 朱立军, 彭运潮, 王坤, 贾杏林, 燕海峰. 黑丝羽乌骨鸡精液DMA颗粒冷冻技术研究[J]. 《中国畜牧兽医》, 2017, 44(2): 447-455.

HUANG Lin, SUN Ao, ZHU Li-jun, PENG Yun-chao, WANG Kun, JIA Xing-lin, YAN Hai-feng. Study on Technology of Black Silkies Chicken Semen Freezing by DMA Pellet[J]. , 2017, 44(2): 447-455.

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黑丝羽乌骨鸡精液DMA颗粒冷冻技术研究

Study on Technology of Black Silkies Chicken Semen Freezing by DMA Pellet

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