绵羊味觉受体第一家族基因外显子多态性分析

doi:10.16431/j.cnki.1671-7236.2016.08.022

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (8): 2081-2094.doi: 10.16431/j.cnki.1671-7236.2016.08.022

绵羊味觉受体第一家族基因外显子多态性分析

轩俊丽^1,2, 马晓萌¹, 袁泽湖¹, 胡师金¹, 王慧华¹, 魏彩虹¹, 赵福平¹, 张莉¹, 杜立新¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 甘肃农业大学动物科学技术学院, 兰州 730070

收稿日期:2016-02-15 出版日期:2016-08-20 发布日期:2016-08-23
通讯作者: 张莉, 杜立新 E-mail:zhangli@263.net;lxdu@263.net
作者简介:轩俊丽(1990-),女,河南太康人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:xuanjunli3566@126.com
基金资助:
“十二五”863项目“绵羊全基因组关联分析研究”（2011AA100307-02）

Polymorphism Analysis of Taste Receptor Family 1 Member Gene Exons in Sheep

XUAN Jun-li^1,2, MA Xiao-meng¹, YUAN Ze-hu¹, HU Shi-jin¹, WANG Hui-hua¹, WEI Cai-hong¹, ZHAO Fu-ping¹, ZHANG Li¹, DU Li-xin¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received:2016-02-15 Online:2016-08-20 Published:2016-08-23

摘要/Abstract

摘要：

为了探讨绵羊味觉受体第一家族（taste receptor family 1 member，T1R）基因外显子多态性及其基因型在乌珠穆沁羊和湖羊群体中分布的差异性，试验采用DNA池直接测序及飞行时间质谱（MALDI-TOFMS）法对中国蒙古系两个绵羊品种共172个个体T1Rs基因外显子的遗传变异情况进行分析，利用生物信息学软件预测多态位点对T1Rs基因mRNA二级结构和蛋白质二级结构的影响。结果表明，在两个群体的T1R家族基因中筛查到9个SNPs。独立性卡方检验显示有5个多态位点基因型的分布在两个绵羊群体中存在显著性差异（P<0.05），分别为TAS1R1基因上的SNP2，TAS1R2基因上的SNP4、SNP7和SNP8，TAS1R3上的SNP10，其中，SNP2、SNP7和SNP10为同义突变；SNP2和SNP10导致相应基因mRNA二级结构和最小自由能的改变，而SNP7仅导致TAS1R2基因最小自由能发生改变；SNP4和SNP8为错义突变，分别导致TAS1R2蛋白质中第379位天冬酰胺变为丝氨酸和第701位苏氨酸变成蛋氨酸，且突变前后受体蛋白的二级结构均发生改变。

关键词: 绵羊; 味觉受体基因; 多态性; 生物信息学

Abstract:

This study was aimed to investigate the distribution of genetic polymorphism of taste receptor family 1 member (T1R) gene between Ujimqin sheep and Hu sheep.DNA pools direct sequencing method and MALDI-TOFMS method were used to analyze genetic variation of T1R genes in 172 sheep of two Chinese sheep strains of Mongolian,and bioinformatics software predicted what impact polymorphic loci had to mRNA and protein secondary structure of T1R gene.The results showed that 9 SNPs were screened in T1R gene of two groups.Chi-square test for independence was taken to find the genotypes of the 5 SNPs which were significantly different between two sheep population(P<0.05),SNP2 located in TAS1R1 gene,SNP4,SNP7 and SNP8 located in TAS1R2 gene,SNP10 located in TAS1R3 gene.SNP2,SNP7 and SNP10 were silent mutations.SNP2 and SNP10 lead to corresponding gene mRNA secondary structure and the minimum free energy change,while the SNP7 only lead to the minimum free energy changes.SNP4 and SNP8 were missense mutations,the two missense mutations respectively led to asparagine(Asn) into serine (Ser),threonine (Thr) into methionine (Met),and according to online software forecast,the protein secondary structure of TAS1R2 gene all changed in mutations before and after.

Key words: sheep; taste receptor gene; polymorphism; boinformatics

中图分类号:

S813.3

轩俊丽, 马晓萌, 袁泽湖, 胡师金, 王慧华, 魏彩虹, 赵福平, 张莉, 杜立新. 绵羊味觉受体第一家族基因外显子多态性分析[J]. 《中国畜牧兽医》, 2016, 43(8): 2081-2094.

XUAN Jun-li, MA Xiao-meng, YUAN Ze-hu, HU Shi-jin, WANG Hui-hua, WEI Cai-hong, ZHAO Fu-ping, ZHANG Li, DU Li-xin. Polymorphism Analysis of Taste Receptor Family 1 Member Gene Exons in Sheep[J]. , 2016, 43(8): 2081-2094.

参考文献

[1] El-Sohemy A,Stewart L,Khataan N,et al.Nutrigenomics of taste-impact on food preferences and food production[J].Forum Nutr,2007,60:176-182.
[2] Garcia-Bailo B,Toguri C,Eny K M,et al.Genetic variation in taste and its influence on food selection[J].OMICS,2009,13(1):69-80.
[3] Roura1 E,Baldwin M W,Klasing K C,et al.禽类味觉系统研究进展[J].中国家禽,2012,34(12):44-47.
[4] 张根华,邓少平.味觉受体第一家族与味觉识别[J].生命的化学,2005,25(3):179-181.
[5] 王兴亚,庞广昌.哺乳动物味觉感受机制研究进展[J].四川动物,2014,33(5):785-791.
[6] Kuhn C,Meyerhof W.Oligomerization of sweet and bitter taste receptors[J].Methods Cell Biol,2013,117:229-242.
[7] Roper S D.Taste buds as peripheral chemosensory processors[J].Semin Cell Dev Biol,2013,24(1):71-79.
[8] Chaudhari N,Roper S D.The cell biology of taste[J].J Cell Biol,2010,190(3):285-296.
[9] Roper S D.Taste buds as peripheral chemosensory processors[J].Semin Cell Dev Biol,2013,24(1):71-79.
[10] Jiang P,Josue-Almqvist J,Jin X,et al.The bamboo-eating giant panda (Ailuropoda melanoleuca) has a sweet tooth:Behavioral and molecular responses to compounds that taste sweet to humans[J].PLoS One,2014,9(3):e93043.
[11] Bassoli A,Borgonovo G,Caremoli F,et al.The taste of D-and L-amino acids:In vitro binding assays with cloned human bitter (TAS2Rs) and sweet (TAS1R2/TAS1R3) receptors[J].Food Chem,2014,150:27-33.
[12] Tordoff M G,Alarcón L K,Valmeki S,et al.T1R3:A human calcium taste receptor[J].Sci Rep,2012,2:496.
[13] Margolskee R F,Dyer J,Kokrashvili Z,et al.T1R3 and gustducin in gut sense sugars to regulate expression of Na⁺-glucose cotransporter1[J].Proc Natl Acad Sci USA,2007,104(38):15075-15080.
[14] Moran A W,Al-Rammahi M,Zhang C,et al.Sweet taste receptor expression in ruminant intestine and itsactivation by artificial sweeteners to regulate glucose absorption[J].J Dairy Sci,2014,97(8):4955-4972.
[15] Damak S,Rong M,Yasumatsu K,et al.Detection of sweet andumami taste in the absence of taste receptor T1R3[J].Science,2003,301(5634):850-853.
[16] Zhao G Q,Zhang Y,Hoon M A,et al.The receptors for mammalian sweet and umami taste[J].Cell,2003,115(3):255-266.
[17] Shi P,Zhang J.Contrasting modes of evolution between vertebrate sweet/umami receptor genes and bitter receptor genes[J].Mol Biol Evol,2006,23(2):292-300.
[18] Hashiguchi Y,Furuta Y,Kawahara R,et al.Diversification and adaptive evolution of putative sweet taste receptors in threespine stickleback[J].Gene,2007,396(1):170-179.
[19] Zhao H,Zhou Y,Pinto C M,et al.Evolution of the sweet taste receptor gene TAS1R2 in bats[J].Mol Biol Evol,2010,27(11):2642-2650.
[20] Zhao H,Yang J R,Xu H,et al.Pseudogenization of the umami taste receptor gene TAS1R1 in the giantpanda coincided with its dietary switch to bamboo[J].Mol Biol Evol,2010,27(12):2669-2673.
[21] Niimura Y,Nei M.Evolutionary dynamics of olfactory and other chemosensory receptor genes in vertebrates[J].J Hum Genet,2006,51(6):505-517.
[22] Li X,Li W,Wang H,et al.Pseudogenization of a sweet-receptor gene accounts for cats' indifference toward sugar[J]. PLoS Genet,2005,1(1):27-35.
[23] 杨嵩,沈峻宇,陈定超,等.家兔TAS1R2基因与生长发育性状的关联性分析[J].四川畜牧兽医,2015,2015(3):29-31.
[24] 李俊,张勇,杨红,等.从江香猪S1M1基因SNPs筛查及生物信息学分析[J].中国畜牧兽医,2015,42(9):2224-2232.
[25] 曲娟娟,于敏,董蕾,等.SNP在动植物遗传育种中的应用[J].东北农业大学学报,2011,42(12):9-13.
[26] 李树珍,万慧荣,杨光.DNA池结合DHPLC和直接测序技术在江豚SNPs检测中的应用[J].兽类学报,2009,29(2):185-190.
[27] 宋桃伟,蔡惠芬,罗卫星.两个贵州山羊品种GHSR和GHRL基因遗传变异及其与生长性状的关联[J].中国农业科学,2015,48(1):140-153.
[28] Liu B,Ha M,Meng X Y,et al.Molecular mechanism of species-dependent sweet taste toward artificial sweeteners[J].J Neurosci,2011,31(30):11070-11076.
[29] Liu Y,Chu A,Chakroun I,et al.Cooperation between myogenic regulatory factors and SIX family transcription factors is important for myoblast differentiation[J].Nucleic Acids Res,2010,38(20):6857-6871.
[30] Omar R L,Arturo P,Erika M L,et al.Sweet taste receptor TAS1R2 polymorphism(Val191Val) is associated with a higher carbohydrate intake and hypertriglyceridemia among the population of West Mexico[J].Nutrients,2016,8(2):E101.
[31] Andre G D,Karen M E,Moira C,et al.Variation in the TAS1R2 gene,sweet taste perception and intake of sugars[J].Journal of Nutrigenetics & Nutrigenomics,2015,8(2):81-90.
[32] Zhang C L,Yuan J,Wang Q,et al.The association of bovine T1R family of receptors polymorphisms with cattle growth traits[J].Res Vet Sci,2012,93(2):783-787.

绵羊味觉受体第一家族基因外显子多态性分析

Polymorphism Analysis of Taste Receptor Family 1 Member Gene Exons in Sheep

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