亚洲黑熊(Ursusthibetanus)血型检测

doi:10.16431/j.cnki.1671-7236.2016.07.029

《中国畜牧兽医》---唯一指定的官方网站 ›› 2016, Vol. 43 ›› Issue (7): 1850-1855.doi: 10.16431/j.cnki.1671-7236.2016.07.029

亚洲黑熊(Ursusthibetanus)血型检测

李勤¹, 陈玉村², 赵俸涌^1,3, 张嘉敏¹, 张树会⁴, 朱自严¹

1. 上海市血液中心, 上海 200051;
2. 福州大熊猫研究中心, 福州 350001;
3. 华东师范大学生命科学学院, 上海 200241;
4. 泉州市泉港东宝养殖有限公司, 泉州 362100

收稿日期:2015-12-21 出版日期:2016-07-20 发布日期:2016-07-22
通讯作者: 朱自严 E-mail:zhuziyan@sbc.org.cn
作者简介:李勤(1980-),男,上海人,硕士,副研究员,研究方向:免疫血液学和动物血型,E-mail:liqin@sbc.org.cn
基金资助:
卫生行业科研专项（201002005）

Blood Typing of Asiatic Black Bear (Ursusthibetanus)

LI Qin¹, CHEN Yu-cun², ZHAO Feng-yong^1,3, ZHANG Jia-min¹, ZHANG Shu-hui⁴, ZHU Zi-yan¹

1. Shanghai Blood Center, Shanghai 200051, China;
2. Fuzhou Giant Panda Research Center, Fuzhou 350001, China;
3. School of Life Science, East China Normal University, Shanghai 200241, China;
4. Quangang Dongbao Animal Breeding Co., Ltd., Quanzhou 362100, China

Received:2015-12-21 Online:2016-07-20 Published:2016-07-22

摘要/Abstract

摘要：

本试验旨在分析亚洲黑熊（Ursusthibetanus）是否具有不同血型。采集中国福建省的40只亚洲黑熊全血样本，使用试管法检测黑熊的血型抗原及血型抗体，抗体的同种型使用2-巯基乙醇鉴定，并对黑熊血浆进行质谱分析。4只黑熊的血浆中检出抗体，该抗体可能是IgM型，可凝集30只亚洲黑熊的红细胞。10只亚洲黑熊血液使用人类血型试剂检测，4只黑熊具有类似人类O型血型，6只具有类似人类B型血型。质谱结果显示，黑熊血清蛋白与大熊猫（Ailuropodamelanoleuca）血清类白蛋白同型异构体1（serum albumin-like isoform 1）有很高同源性。上述结果提示，亚洲黑熊具有至少1个血型系统，该系统至少具有2种血型。如果患病黑熊需要接受输血治疗，交叉配血试验是必需的。此外，在紧急情况下，大熊猫可接受亚洲黑熊血液。

关键词: 亚洲黑熊; 血型系统; 交叉配血试验; 输血医学

Abstract:

In this study,we aimed to determine whether bear had different blood group systems.Whole blood samples were collected from 40 Asiatic Black bears in Fujian province,China.Tube method was used to detect antibodies in plasma,and antibody isotype was determined with 2-mercaptoethanol.Plasma was further analyzed by mass spectrometry.The plasma from four bears had antibodies,possibly IgM isoform,which could agglutinate RBCs from 30 bears.Blood samples from 10 bears were tested by human blood typing reagents.The results showed that 4 bears had blood type like human type O,while 6 bears had like human type B.Plasma protein had extensive homology to serum albumin-like isoform 1 found in giant panda (Ailuropodamelanoleuca).We suggested that Asiatic Black bear might have at least one blood group system with two blood types.If the sick bear needs blood transfusion,a cross-matching test was necessary.Moreover,giant panda might receive blood from Asiatic Black bear in case of emergency.

Key words: Asiatic Black bear (Ursusthibetanus); blood group system; cross-matching test; transfusion medicine

中图分类号:

S852.5

李勤, 陈玉村, 赵俸涌, 张嘉敏, 张树会, 朱自严. 亚洲黑熊(Ursusthibetanus)血型检测[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2016, 43(7): 1850-1855.

LI Qin, CHEN Yu-cun, ZHAO Feng-yong, ZHANG Jia-min, ZHANG Shu-hui, ZHU Zi-yan. Blood Typing of Asiatic Black Bear (Ursusthibetanus)[J]. , 2016, 43(7): 1850-1855.

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亚洲黑熊(Ursusthibetanus)血型检测

Blood Typing of Asiatic Black Bear (Ursusthibetanus)

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