原花青素对玉米赤霉烯酮致小鼠肝脏、肾脏氧化损伤的保护作用

doi:10.16431/j.cnki.1671-7236.2016.02.017

摘要/Abstract

摘要： 试验旨在研究原花青素(PC)是否对玉米赤霉烯酮(ZEA)中毒的小鼠肝脏及肾脏具有保护作用.选取40只日龄为8~9周、体重约为45 g的健康清洁级雄性昆明系小鼠,随机分为4组,对照组灌喂生理盐水,PC组灌喂100 mg/kg PC,ZEA组灌喂40 mg/kg ZEA,PC＋ZEA组灌喂100 mg/kg PC+40 mg/kg ZEA,眼球采血,颈椎处死后采取肝脏样本.测定肝脏组织中谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量及血清中尿酸(UA)、尿素氮(BUN)含量.结果显示,与对照组相比,PC组各项指标差异均不显著(P>0.05),提示肝脏及肾脏没有明显的氧化损伤;与对照组相比,ZEA组的小鼠组织中谷草转氨酶、谷丙转氨酶及丙二醛含量显著或极显著升高(P<0.05;P<0.01),超氧化物歧化酶含量极显著降低(P<0.01),血清中尿酸、尿素氮含量极显著升高(P<0.01),提示肝脏及肾脏有严重的氧化损伤;PC+ZEA组的小鼠各项指标(除超氧化物歧化酶外)均显著或极显著低于ZEA组(P<0.05;P<0.01),提示其肝脏及肾脏氧化损伤程度低于ZEA组,PC对ZEA中毒小鼠的肝脏及肾脏氧化损伤有一定的保护作用.

关键词: 原花青素; 玉米赤霉烯酮; 氧化损伤; 保护作用

Abstract: The experiment was conducted to study whether the proanthocyanidin (PC) had the protective effects on the liver and kidney oxidative damage induced by zearalenone in mice.40 8 to 9 week-old healthy clean grade Kunming mice with the 45 g body weight were chosen and randomly divided into four groups: control group was given normal saline,PC group was given 100 mg/kg PC,ZEA group was given 40 mg/kg ZEA and PC＋ZEA group was fed 100 mg/kg PC＋40 mg/kg ZEA.Liver and blood sampls were taken after cervical vertebra to death.AST、ALT、MDA、SOD were measured to determine the degree of liver damage and antioxidant capacity,and UA and BUN were used to determine the kidney damage.The results showed that: Compared with the control group,the indexes of PC group were not significant changed (P>0.05),indicating that there was no obvious oxidative damage in the liver and kidney.Compared with control group,ALT,MDA and AST content were significantly or extremely significant increased in ZEA group (P<0.05;P<0.01),SOD content was extremely significant decreased (P<0.01),UA and BUN were extremely significant increased in serum (P<0.01),indicating that the liver and kidney of ZEA group had a serious oxidative damage.The indexes of the PC＋ZEA group (except SOD)were significantly or extremely significant lower than ZEA group (P<0.05;P<0.01),indicating that the oxidative damage of the liver and kidney in PC＋ZEA group was lower than that in the ZEA group.In conclusion,the liver and kidney oxidative damage of mice induced by ZEA was eased to some extent by PC.

Key words: proanthocyanidins; zearalenone; oxidative damage; protection

中图分类号:

S816

韩建鑫, 何剑斌, 高锋, 杨淑华, 梁甜甜, 董双, 张燚, 龙淼. 原花青素对玉米赤霉烯酮致小鼠肝脏、肾脏氧化损伤的保护作用[J]. , 2016, 43(2): 402-406.

HAN Jian-xin, HE Jian-bin, GAO Feng, YANG Shu-hua, LIANG Tian-tian, DONG Shuang, ZHANG Yi, LONG Miao. Protective Effects of Proanthocyanidins on the Liver and Kidney Oxidative Damage Induced by Zearalenone in Mice[J]. , 2016, 43(2): 402-406.

参考文献

[1] 季海霞,苏永腾.2014年饲料霉菌毒素分析报告[J].广东饲料,2015,24(2):39-41.
[2] 姜淑贞,杨维仁,杨在宾.玉米赤霉烯酮的污染和残留及其作用机制[J].中国饲料,2011,2:41-44.
[3] 黄丽娜,彭双清,刘宁.玉米赤霉烯酮生殖发育毒性的研究进展[J].中国地方病防治杂志,2014,3:181-184.
[4] 王青,周丹朝,赵煜,等.玉米赤霉烯酮的生殖毒性研究进展[J].畜牧兽医杂志,2014,33(4):32-35.
[5] 范小龙,刘长永,剡海阔,等.玉米赤霉烯酮对小鼠脾淋巴细胞增殖及分泌细胞因子的影响[J].中国畜牧兽医,2011,38(5):48-54.
[6] 范小龙,许利娜,剡海阔,等.玉米赤霉烯酮对小鼠胸腺上皮细胞线粒体的影响[J].中国畜牧兽医,2011,38(6):41-45.
[7] 梁梓森,马勇江,刘长永,等.玉米赤霉烯酮对小鼠免疫器官的毒性作用[J].中国兽医科学,2010,40(3):279-283.
[8] 赵建华,邹杨,姜淑贞.玉米赤霉烯酮毒害作用研究进展[J].山东畜牧兽医,2008,139(8):45-47.
[9] Kang C,Lee H,Yoo Y S,et al.Evaluation of oxidative DNA damage using an alkaline single cell gel electroPhoresis (SCGE) comet assay,and the protective effects of N-Acetylcysteine amide on zearalenone-induced cytotoxicity in Chang liver cells[J].Toxicological Research,2013,29(1):43-52.
[10] 张妍,吴秀香.原花青素研究进展[J].中药药理与临床,2011,6:112-116.
[11] Nishizuka T,Fujita Y,Sato Y,et al.Procyanidins are potent inhibitors of LOX-1:A new player in the French Paradox[J].Proc Jpn Acad Ser B Phys Biol Sci,2011,87(3):104-113.
[12] Zhang H,Yerigui,Yang Y,et al.Structures and antioxidant and intestinal disaccharidase inhibitory activities of A-type proanthocyanidins from peanut skin[J].Journal of Agricultural and Food Chemistry,2013,61(37):8814-8820.
[13] Belcaro G,Ledda A,Hu S,et al.Grape seed procyanidins in pre-and mild hypertension:A registry study[J].Evid Based Complement Alternat Med,2013,2013:1-5.
[14] Serra A T,Rocha J,Sepodes B,et al.Evaluation of cardiovascular protective effect of different apple varieties-correlation of response with composition[J].Food Chemistry,2012,135(4):2378-2386.
[15] Fracassetti D,Costa C,Moulay L,et al.Ellagic acid derivatives,ellagitannins,proanthocyanidins and other phenolics,vitamin C and antioxidant capacity of two powder products from camu-camu fruit (Myrciaria dubia)[J].Food Chemistry,2013,139(1-4):578-588.
[16] 陈欢,马磊,黄茹,等.原花青素在对乙酰氨基酚诱导的急性肝损伤中的保护作用及机制[J].重庆医科大学学报,2015,40(3):358-361.
[17] 高峰,张琨,宋昕恬,等.葡萄籽提取物抗氧化作用人体实验研究[J].中国卫生工程学,2010,2:99-100.
[18] 梁梓森,马勇江,刘长永,等.玉米赤霉烯酮对小鼠肝脏及肾脏的毒性作用[J].中国兽医学报,2010,5:673-676.
[19] 梁梓森,刘长永,马勇江,等.玉米赤霉烯酮对小鼠血常规及血液生化指标的影响[J].动物医学进展,2010,31(5):79-82.
[20] Kouadio J H,Mobio T A,Baudrimont I,et al.Comparative study of cytotoxicity and oxidative stress induced by deoxynivalenol,zearalenone or fumonisin B1 in human intestinal cell line Caco-2[J].Toxicology,2005,213(1-2):56-65.
[21] 张娣.葡萄籽中原花青素抗氧化效果研究进展[J].黑龙江生态工程职业学院学报,2014,2:17-18.
[22] Jiang S Z,Yang Z B,Yang W R,et al.Effect on hepatonephric organs,serum metabolites and oxidative stress in post-weaning piglets fed purified zearalenone-contaminated diets with or without Calibrin-Z[J].Journal of Animal Physiology and Animal Nutrition,2011,96(6):1147-1156.
[23] Lee H,Kang C,Yoo Y S,et al.Cytotoxicity and the induction of the stress protein Hsp 70 in Chang liver cells in response to zearalenone-induced oxidative stress[J].Environmental Toxicology and Pharmacology,2013,36(2):732-740.
[24] Zourgui L,Golli E E,Bouaziz C,et al.Cactus (Opuntia ficus-indica) cladodes prevent oxidative damage induced by the mycotoxin zearalenone in Balb/C mice[J].Food and Chemical Toxicology,2008,46(5):1817-1824.
[25] Marin D E,Pistol G C,Neagoe I V,et al.Effects of zearalenone on oxidative stress and inflammation in weanling piglets[J].Food and Chemical Toxicology,2013,58:408-415.
[26] Jia Z,Liu M,Qu Z,et al.Toxic effects of zearalenone on oxidative stress,inflammatory cytokines,biochemical and pathological changes induced by this toxin in the kidney of pregnant rats[J].Environmental Toxicology and Pharmacology,2014,37(2):580-591.