猪接触传染性胸膜肺炎放线杆菌血清3型分子鉴定及药敏试验

doi:10.16431/j.cnki.1671-7236.2016.01.041

›› 2016, Vol. 43 ›› Issue (1): 280-284.doi: 10.16431/j.cnki.1671-7236.2016.01.041

• 疾病防治 • 上一篇

猪接触传染性胸膜肺炎放线杆菌血清3型分子鉴定及药敏试验

李海利¹, 徐引弟¹, 宋毓民², 朱文豪¹, 张青娴¹, 王克领¹, 冯亚杰¹, 候自花¹

1. 河南省农业科学院畜牧兽医研究所, 郑州 450002;
2. 山东省临沂市兰山区畜牧兽医局, 临沂 276000

收稿日期:2015-07-28 出版日期:2016-01-20 发布日期:2016-01-23
通讯作者: 王克领 E-mail:344601509@qq.com
作者简介:李海利(1982-),男,河南开封人,博士,助理研究员,研究方向:动物传染病临床诊断及防控,E-mail:haili8693@sina.com
基金资助:
河南省农业科学院优秀青年科技基金(2013YQ22);河南省农业科学院自主创新基金

Molecular Identification and Antimicrobial Susceptibility Test of Porcine Contagious Actinobacillus pleuropneumoniae Serotype 3

LI Hai-li¹, XU Yin-di¹, SONG Yu-min², ZHU Wen-hao¹, ZHANG Qing-xian¹, WANG Ke-ling¹, FENG Ya-jie¹, HOU Zi-hua¹

1. Institute of Animal Husbandry and Veterinary Research, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
2. Lanshan Bureau of Animal Husbandry and Veterinary, Linyi 276000, China

Received:2015-07-28 Online:2016-01-20 Published:2016-01-23

摘要/Abstract

摘要： 为了解猪接触传染性胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)的血清型及耐药情况,本研究根据GenBank数据库设计1对引物,特异性的扩增950 bp核苷酸片段,对血清3型APP进行分子鉴定及药敏试验。结果显示,所得PCR产物经过测序,与GenBank已发表的血清3型APP的同源性达99%以上,所分离菌株耐药性较强,大多为多重耐药。通过对血清3型APP进行分子鉴定及药敏试验,为猪传染性胸膜肺炎的鉴定、诊断及防制提供了基础。

关键词: 猪传染性胸膜肺炎; 胸膜肺炎放线杆菌; 血清3型; 药敏试验

Abstract: In order to understand the serotype and drug resistance of porcine contagious Actinobacillus pleuropneumoniae(APP), a pair of primers was designed according to GenBank database to amplify specific 950 bp fragment, and the molecular identification and antimicrobial susceptibility test of APP serotype 3 were investigated.The results showed that the PCR product sequences were more than 99% homology with the APP serotype 3 published in GenBank.The isolated strains were highly resistant and multiple drug resistance.The molecular identification and antimicrobial susceptibility test of APP serotype 3 provided the basis for the identification, diagnosis and prevention of porcine contagious pleuropneumonia.

Key words: porcine contagious pleuropneumonia; Actinobacillus pleuropneumoniae; serotype 3; antimicrobial susceptibility test

中图分类号:

S852.61⁺9

李海利, 徐引弟, 宋毓民, 朱文豪, 张青娴, 王克领, 冯亚杰, 候自花. 猪接触传染性胸膜肺炎放线杆菌血清3型分子鉴定及药敏试验[J]. , 2016, 43(1): 280-284.

LI Hai-li, XU Yin-di, SONG Yu-min, ZHU Wen-hao, ZHANG Qing-xian, WANG Ke-ling, FENG Ya-jie, HOU Zi-hua. Molecular Identification and Antimicrobial Susceptibility Test of Porcine Contagious Actinobacillus pleuropneumoniae Serotype 3[J]. , 2016, 43(1): 280-284.

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猪接触传染性胸膜肺炎放线杆菌血清3型分子鉴定及药敏试验

Molecular Identification and Antimicrobial Susceptibility Test of Porcine Contagious Actinobacillus pleuropneumoniae Serotype 3

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