广东地区水禽源大肠杆菌对β-内酰胺类药物的耐药性及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2015.09.040

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (9): 2487-2492.doi: 10.16431/j.cnki.1671-7236.2015.09.040

广东地区水禽源大肠杆菌对β-内酰胺类药物的耐药性及耐药基因检测

张济培¹, 谭华龙¹, 韦庆兰², 陈建红¹

1. 佛山科学技术学院生命科学学院, 佛山 528231;
2. 华南农业大学兽医学院, 广州 510642

收稿日期:2015-04-01 出版日期:2015-09-20 发布日期:2015-09-25
通讯作者: 陈建红 E-mail:chenjianhong2012@126.com
作者简介:张济培(1970-),男,广东陆河人,硕士,高级实验师,研究方向:预防兽医学,E-mail:fszh128@163.com
基金资助:
广东省产业技术研究与开发专项资金项目(2013B020307018)

Detection of Resistance and Resistance Genes of Escherichia coli to β-lactams Antimicrobial Agents Isolated from Waterfowl in Guangdong Province

ZHANG Ji-pei¹, TAN Hua-long¹, WEI Qing-lan², CHEN Jian-hong¹

1. College of Life Sciences, Foshan University, Foshan 528231, China;
2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

Received:2015-04-01 Online:2015-09-20 Published:2015-09-25

摘要/Abstract

摘要： 为了调查广东地区水禽源大肠杆菌对β-内酰胺类抗菌药的耐药性和耐药基因的流行情况,本试验于2012—2014年从广东地区患病水禽中成功分离鉴定243株大肠杆菌,采用琼脂二倍稀释法测定大肠杆菌分离株对4种β-内酰胺类药物的敏感性,并通过PCR方法检测耐药株的耐药基因bla_CTX-M、bla_TEM和bla_OXA。药敏试验结果显示,广东各地区的分离株对氨苄西林和阿莫西林耐药率较高,耐药率为80.00%~100.00%;而对头孢他啶和头孢噻呋耐药率较低,耐药率为9.09%~50.00%。PCR检测结果显示,在4种药物的耐药菌株中,耐药基因bla_CTX-M、bla_TEM和bla_OXA的检出率分别为88.46%~90.87%、67.21%~88.52%和51.28%~59.02%。结果表明,水禽大肠杆菌对β-内酰胺类药物普遍具有耐药性,其中青霉素类尤为严重。bla_CTX-M、bla_TEM和bla_OXA基因为广东地区水禽源大肠杆菌携带的主要耐药基因,且耐药基因的普遍存在与临床日益严重的耐药现象有着很大的关系。

关键词: 水禽大肠杆菌; 药敏试验; β -内酰胺类耐药基因

Abstract: In order to understand the resistance to β-lactams antimicrobial agents and the prevalence of resistance genes, 243 Escherichia coli (E.coli) were isolated and identified from waterfowl in Guangdong province during 2012 to 2014.Drug sensitivity of total 243 E.coli isolates to 4 kinds of β-lactams antimicrobial agents were determined by double broth dilution method.All β-lactams resistant isolates were detected for the presences of bla_CTX-M, blasub>TEM and bla_OXA genes by PCR.The results showed that the occurrence of ampicillin and amoxicillin were ranked the higher (80.00% to 100.00%), and were 9.09% to 50.00% for ceftazidime and ceftiofur.The bla_CTX-M, bla_TEM and bla_OXA genes were detected in 88.46% to 90.78%, 67.21% to 88.52% and 51.28% to 59.02% of the β-lactams resistant isolates to the four kinds of antimicrobial agents, respectively.The results indicated that the resistance of E.coli isolates from waterfowl to β-lactams antimicrobial agents were widespread, and penicillin was particularly serious.The bla_CTX-M, bla_TEM and bla_OXA genes were mainly carried β-lactams resistant genes in E.coli isolates from waterfowl in Guangdong province.It had a great relationship between the prevalence of resistance genes and growth of antimicrobial resistance.

Key words: Escherichia coli from waterfowl; drug sensitivity test; β-lactams resistance genes

中图分类号:

S852.61⁺2

张济培, 谭华龙, 韦庆兰, 陈建红. 广东地区水禽源大肠杆菌对β-内酰胺类药物的耐药性及耐药基因检测[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(9): 2487-2492.

ZHANG Ji-pei, TAN Hua-long, WEI Qing-lan, CHEN Jian-hong. Detection of Resistance and Resistance Genes of Escherichia coli to β-lactams Antimicrobial Agents Isolated from Waterfowl in Guangdong Province[J]. , 2015, 42(9): 2487-2492.

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广东地区水禽源大肠杆菌对β-内酰胺类药物的耐药性及耐药基因检测

Detection of Resistance and Resistance Genes of Escherichia coli to β-lactams Antimicrobial Agents Isolated from Waterfowl in Guangdong Province

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