不同辐照剂量对蜂球囊菌杀灭效果的影响

doi:10.16431/j.cnki.1671-7236.2015.04.038

›› 2015, Vol. 42 ›› Issue (4): 1021-1027.doi: 10.16431/j.cnki.1671-7236.2015.04.038

不同辐照剂量对蜂球囊菌杀灭效果的影响

董文滨, 刘锋, 李肖, 胥保华

山东农业大学动物科技学院, 泰安 271018

收稿日期:2014-09-17 出版日期:2015-04-20 发布日期:2015-05-05
通讯作者: 胥保华 E-mail:bhxu@sdau.edu.cn
作者简介:董文滨(1988-),男,山东高唐人,硕士生,研究方向:蜜蜂营养与饲料科学,E-mail:dwbsdau@126.com
基金资助:
现代农业蜂产业技术体系建设专项资金(CARS-45);公益性行业(农业)科研专项经费项目(200903006)

Effect of Different Irradiation Dosage on the Killing of Ascophaera apis

DONG Wen-bin, LIU Feng, LI Xiao, XU Bao-hua

College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received:2014-09-17 Online:2015-04-20 Published:2015-05-05

摘要/Abstract

摘要： 本试验以蜂球囊菌(Ascosphaera apis)为研究对象,探究不同辐照剂量对蜂球囊菌的杀灭效果,以期明确有效杀灭蜂球囊菌的最低辐照剂量,为Co60γ射线辐照蜜蜂饲料(特别是蜂花粉)时的辐照剂量选择提供依据.利用细菌纯化技术从患白垩病意蜂幼虫体内分离纯化得到蜂球囊菌,并结合形态学、乳酸酚棉兰染色及5.8S rDNA序列分析技术进行鉴定;同时制备不同梯度浓度的蜂球囊菌孢子悬液加入到蜜蜂幼虫饲料中,以饲喂方式侵染3日龄意蜂幼虫,确定半数致死浓度(LC₅₀);蜜蜂幼虫饲料中添加上述确定LC₅₀的孢子,以不同辐照剂量处理,并通过侵染幼虫试验,确定有效杀灭蜜蜂饲料中孢子的最低辐照剂量.结果表明,通过形态学和分子生物学鉴定,从患白垩病的意蜂幼虫体内分离纯化得到真菌即为蜜蜂白垩病的病原——蜂球囊菌;蜂球囊菌对人工饲养条件下的意蜂幼虫的LC₅₀为9.5×10⁴个/mL;当Co60γ射线辐照剂量为7.0 kGy时,幼虫患病率与未辐照组差异显著(P<0.05),由此确定对添加半数致死浓度的蜂球囊菌孢子饲料的最低有效辐照剂量为7.0 kGy.

关键词: 蜂球囊菌; 辐照剂量; 蜜蜂饲料; 白垩病

Abstract: In order to obtain the lowest irradiation dose on effectively killing Ascosphaera apis, our study took Ascosphaera apis as the research object, and explored the killing effect of different irradiation dose on the Ascosphaera apis. Also, we hoped to provide a reference basis for Co60γ irradiating bee feed (especially for bee pollen). During the study, we had used bacteria-purification technology to obtaine Ascosphaera apis from cretaceous disease (bee chalkbrood diseases) larvae, and combined with morphology, lactic acid phenol medan dyeing and 5.8S rDNA sequence analysis technology to identificate this Ascosphaera apis. In addition, we preparated different levels of Ascosphaera apis spore suspension, and added them to 3 d larvae feed to test the half lethal dose (LC₅₀). Behind, we added LC₅₀ level of spore suspension into larvae diet, and then adopted different irradiation dose irradiation diet, finally fed larvae with that treated diets. The results showed that the isolated and purified fungal pathogen, which got from bee chalkbrood disease larvae, were bee balloon bacteria, identified by morphological and molecular biology. Furthermore, under the condition of indoor artificial breeding, the LC₅₀ of bacteria Ascosphaera apis on larvae was 9.5×10⁴/mL, and the effectively killing dose was 7.0 kGy. There was significant difference between larvae prevalence and the irradiation group (P<0.05). From this we could be sure that the minimum effective radiation dose of bee feed, which was added LC₅₀ balloon fungus spores was 7.0 kGy.

Key words: Ascosphaera apis; irradiation dose; bees feed; chalkbrood disease

中图分类号:

S896

董文滨, 刘锋, 李肖, 胥保华. 不同辐照剂量对蜂球囊菌杀灭效果的影响[J]. , 2015, 42(4): 1021-1027.

DONG Wen-bin, LIU Feng, LI Xiao, XU Bao-hua. Effect of Different Irradiation Dosage on the Killing of Ascophaera apis[J]. , 2015, 42(4): 1021-1027.

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不同辐照剂量对蜂球囊菌杀灭效果的影响

Effect of Different Irradiation Dosage on the Killing of Ascophaera apis

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