关键词: 复州牛; 超数排卵; 有效胚

Abstract: To build a reasonable and valid superovulation scheme,get more superior embryos and conserve the whole genome of Chinese native Fuzhou cattle,47 Fuzhou donor cows were hired to perform superovulation treatment in the present study.The superovulation effects were systemically analyzed and compared from different batch processing of superovulation, different lactation status,different brands and dosages of FSH.The results showed that via superovulating,7.98 recycled eggs per head were collected in the Fuzhou donor cows,of which effective embryos were 4.62.The average number of effective embryos in the third group (8.25) was significantly higher than that in the first (2.00) and the second (2.79) groups (P <0.05).The average number of luteums (15.38),the average number of recycled eggs (7.63) and the average number of effective embryos (5.75) of the cattle not in milk were extremely significantly higher than that of the cattle in milk (10.64,2.18 and 0.64,respectively) (P <0.01). The average number of recycled eggs and the average number of effective embryos of Fuzhou cattle treated with Jp-FSH were 12.70 and 7.50,respectively.The similar superovulation effects were observed in the Fuzhou cattle treated with Ch-FSH (10.62 and 7.38,respectively) (P >0.05).The suitable dose of Ch-FSH was 8.8 mg per head,The average number of effective embryos and the ratio of effective embryos were 7.38 and 69%,respectively.The results indicated that desired superovulation effects were obtained through optimizing the superovulation schemes in Fuzhou cattle.

Key words: Fuzhou cattle; superovulation; effective embryo