猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立

doi:10.11843/j.issn.1671-7236.2015.03.003

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (3): 525-530.doi: 10.11843/j.issn.1671-7236.2015.03.003

猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立

宋晓丽¹, 刘梅轩¹, 罗湘蜀¹, 李成龙¹, 杨术鹏¹, 王战辉^1,2, 张素霞^1,2

1. 中国农业大学动物医学院, 北京 100193;
2. 国家兽药残留基准实验室, 北京 100193

收稿日期:2014-10-14 出版日期:2015-03-20 发布日期:2015-03-30
通讯作者: 张素霞 E-mail:suxia@cau.edu.cn
作者简介:宋晓丽(1988-),女,内蒙古赤峰人,硕士生,研究方向:动物源性食品中兽药残留快速检测方法建立,E-mail:mysongxiaoli@126.com
基金资助:
"973"国家重点基础研究发展计划课题(No.2009CB118801)"受试化合物在畜禽体内的代谢研究"

Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken

SONG Xiao-li¹, LIU Mei-xuan¹, LUO Xiang-shu¹, LI Cheng-long¹, YANG Shu-peng¹, WANG Zhan-hui^1,2, ZHANG Su-xia^1,2

1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
2. National Reference Laboratory for Veterinary Drug Residue, Beijing 100193, China

Received:2014-10-14 Online:2015-03-20 Published:2015-03-30

摘要/Abstract

摘要： 为保障动物源性食品安全,本研究建立了分别检测猪肉和鸡肉中脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和T-2毒素残留的间接竞争酶联免疫吸附法.结果显示,该方法在猪肉和鸡肉样本中DON的检测限分别为34.9和43.5 μg/kg,添加回收率为72.7%~97.1%,变异系数小于8.7%;T-2毒素的检测限分别为33.7和28.7 μg/kg,添加回收率为72.1%~95.0%,变异系数小于11.3%.该方法灵敏度高、准确、简便,适用于猪肉和鸡肉中DON和T-2毒素残留的快速检测.

关键词: 酶联免疫吸附法(ELISA); 脱氧雪腐镰刀菌烯醇(DON); T-2毒素; 猪肉; 鸡肉

Abstract: To protect animal-origin foods,an indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed to detect deoxynivalenol (DON) and T-2 toxin in pork and chicken,respectively.The results showed that the limit of detection (LOD) of DON in pork and chicken were 34.9 and 43.5 μg/kg,respectively,and the recoveries ranged from 72.7% to 97.1% with the coefficients of variation (CV) lower than 8.7%.Besides,the LOD of T-2 toxin in pork and chicken were 33.7 and 28.7 μg/kg,respectively,and the recoveries ranged from 72.1% to 95.0% with CV less than 11.3%.The developed method had the advantage of being sensitive and accurate,and was suitable for the rapid detection of DON and T-2 residue in pork and chicken.

Key words: enzyme-linked immunosorbent assay (ELISA); deoxynivalenol (DON); T-2 toxin; pork; chicken

中图分类号:

R155.5

宋晓丽, 刘梅轩, 罗湘蜀, 李成龙, 杨术鹏, 王战辉, 张素霞. 猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(3): 525-530.

SONG Xiao-li, LIU Mei-xuan, LUO Xiang-shu, LI Cheng-long, YANG Shu-peng, WANG Zhan-hui, ZHANG Su-xia. Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken[J]. , 2015, 42(3): 525-530.

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猪肉和鸡肉中脱氧雪腐镰刀菌烯醇和T-2毒素酶联免疫吸附检测方法的建立

Development of an Enzyme-linked Immunosorbent Assay for the Detection of Deoxynivalenol and T-2 Toxin in Pork and Chicken

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