促性腺激素释放激素及其类似物在母猪繁殖中应用的研究进展

doi:10.16431/j.cnki.1671-7236.2019.02.022

《中国畜牧兽医》 ›› 2019, Vol. 46 ›› Issue (2): 512-518.doi: 10.16431/j.cnki.1671-7236.2019.02.022

促性腺激素释放激素及其类似物在母猪繁殖中应用的研究进展

刘闯^1,5, 李楠¹, 魏巧莉^1,5, 房晓欢^1,5, 田见晖², 翁士乔³, 刘彦⁴, 李俊杰^1,5

1. 河北农业大学动物科技学院, 保定 071000;
2. 中国农业大学动物科技学院, 北京 100193;
3. 宁波三生生物科技有限公司, 宁波 315000;
4. 北京市农林科学院畜牧兽医研究所, 北京 100097;
5. 河北省牛羊胚胎工程技术研究中心, 保定 071000

收稿日期:2018-08-07 出版日期:2019-02-20 发布日期:2019-02-20
通讯作者: 李俊杰 E-mail:lijunjie816@163.com
作者简介:刘闯(1991-),男,河南信阳人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:335674341@qq.com
基金资助:
"十三五"国家重点研发计划课题（2017YFD0501901）；河北省现代农业产业体系专项资金（HBCT2018110201）

Research Progress on Application of Gonadotropin-releasing Hormone and Its Analogues in Porcine Reproduction

LIU Chuang^1,5, LI Nan¹, WEI Qiaoli^1,5, FANG Xiaohuan^1,5, TIAN Jianhui², WENG Shiqiao³, LIU Yan⁴, LI Junjie^1,5

1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
2. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
3. Ningbo Sansheng Biological Technology Co., Ltd., Ningbo 315000, China;
4. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
5. Research Center of Cattle and Sheep Embryo Engineering Technique of Hebei, Baoding 071000, China

Received:2018-08-07 Online:2019-02-20 Published:2019-02-20

摘要/Abstract

摘要：

促性腺激素释放激素（gonadotropin-releasing hormone，GnRH））是下丘脑分泌的生殖激素，主要通过下丘脑-垂体-性腺轴参与调控动物的生殖活动，也可直接作用于性腺或其他器官发挥重要功能。哺乳动物的GnRH具有相同的十肽结构，通过改变十肽结构中第六、九、十位氨基酸可合成不同的GnRH类似物。GnRH及其类似物可通过刺激促黄体素（LH）分泌、抑制雌激素受体二聚化及调节胚胎附植期相关蛋白质的合成来影响动物的繁殖性能。GnRH及其类似物已被证明可提高猪的繁殖力。在母猪生产中，GnRH类似物的应用仍存在受胎次影响、促进排卵但不能增加产仔数等问题。文章主要从GnRH的来源与功能、GnRH及其类似物的结构、GnRH受体（GnRHR）的结构与功能、GnRH及其类似物对母猪繁殖性能的影响，以及存在的问题与展望五方面介绍了GnRH及其类似物在母猪繁殖中的应用研究进展。

关键词: 促性腺激素释放激素(GnRH); 促性腺激素释放激素类似物; 母猪繁殖

Abstract:

Gonadotropin-releasing hormone (GnRH) is a reproductive hormone secreted by the hypothalamus.It mainly participates in regulating the reproductive activities of animals through the hypothalamic-pituitary-gonadal axis,and can also directly play an important role in animal gonads or other organs.GnRH in mammals has the same ten peptide structure.Different GnRH analogues can be synthesized by changing the sixth,ninth and tenth amino acids in the ten peptide structure.GnRH and its analogues can affect animal reproductive performance by stimulating luteinizing hormone (LH) secretion,inhibiting estrogen receptor dimerization and regulating the synthesis of proteins associated with embryo implantation.GnRH and its analogues in production have been shown to increase the fertility of pigs.The application of GnRH analogues still has some problems,such as the significant effect of the second pregnancy,the promotion of ovulation but not the increase of litter size.This paper introduced the application of GnRH and its analogues in sow breeding from such aspects:The source and function of GnRH,the structure of GnRH and its analogues,the structure and function of gonadotropin-releasing hormone receptor,the effect of GnRH and its analogues on reproductive performance of swine,existing problems and prospects.

Key words: gonadotropin-releasing hormone (GnRH); GnRH analogues; porcine reproduction

中图分类号:

S828

刘闯, 李楠, 魏巧莉, 房晓欢, 田见晖, 翁士乔, 刘彦, 李俊杰. 促性腺激素释放激素及其类似物在母猪繁殖中应用的研究进展[J]. 《中国畜牧兽医》, 2019, 46(2): 512-518.

LIU Chuang, LI Nan, WEI Qiaoli, FANG Xiaohuan, TIAN Jianhui, WENG Shiqiao, LIU Yan, LI Junjie. Research Progress on Application of Gonadotropin-releasing Hormone and Its Analogues in Porcine Reproduction[J]. , 2019, 46(2): 512-518.

参考文献

[1] KNOX R V,TAIBL J N,BREEN S M,et al.Effects of altering the dose and timing of triptorelin when given as an intravaginal gel for advancing and synchronizing ovulation in weaned sows[J].Theriogenology,2014,82(3):379-386.
[2] KNOX R V,ESPARZA-HARRIS K C,JOHNSTON M E,et al.Effect of numbers of sperm and timing of a single,post-cervical insemination on the fertility of weaned sows treated with OvuGel?[J].Theriogenology,2017,92(33):197-203.
[3] DE JONG E,KAUFFOLD J,ENGL S,et al.Effect of a GnRH analogue (Maprelin) on the reproductive performance of gilts and sows[J].Theriogenology,2013,80(8):870-877.
[4] 刘斌,刘通,郑鹏.利用激素处理提高大日龄不发情后备母猪的繁殖能力[J].黑龙江畜牧兽医,2017,2:75-77. LIU B,LIU T,ZHEN P.Using hormone treatment to improve the reproductive capacity of non-estrus gilts[J].Heilongjiang Animal Science and Veterinary Medicine,2017,2:75-77.(in Chinese)
[5] DE JONG E,JOURQUIN J,KAUFFOLD J,et al.Effect of a GnRH analogue (Peforelin) on the litter performance of gilts and sows[J].Porcine Health Management,2017,3(1):54-60.
[6] MOORE JR J P,WRAY S.Luteinizing hormone-releasing hormone (LHRH) biosynthesis and secretion in embryonic LHRH neurons[J]. Endocrinology,2000,141(12):4486-4495.
[7] KIRKWOOD R N,LAPWOOD K R,SMITH W C,et al.Plasma concentrations of LH,prolactin,oestradiol-17β and progesterone in sows weaned after lactation for 10 or 35 days[J].Journal of Reproduction and Fertility,1984,70(1):95-102.
[8] SKINNER D C,ALBERTSON A J,NAVRATIL A,et al.Effects of gonadotrophin-releasing hormone outside the hypothalamic-pituitary-reproductive axis[J].Journal of Neuroendocrinology,2009,21(4):282-292.
[9] YE R S,XI Q Y,QI Q,et al.Differentially expressed miRNAs after GnRH treatment and their potential roles in FSH regulation in porcine anterior pituitary cell[J].PLoS One,2013,8(2):e57156.
[10] 丁赫,宫永胜,王军,等.初情期启动过程中小尾寒羊下丘脑GnRH基因甲基化状态及表达量关系研究[J].中国畜牧杂志,2018,54(5):58-61. DING H,GONG Y S,WANG J,et al.Relationship between methylation status and expression of GnRH gene in Small Tail Han sheep during onset of pu-berty[J].Chinese Journal of Animal Science,2018,54(5):58-61.(in Chinese)
[11] KOVACS M,SCHALLY A V,NAGY A,et al.Recovery of pituitary function after treatment with a targeted cytotoxic analog of luteinizing hormone-releasing hormone[J]. Proceedings of the National Academy of Sciences,1997,94(4):1420-1425.
[12] KAYA D,GRAM A,KOWALEWSKI M P,et al.Expression of GnRH receptor in the canine corpus luteum,and luteal function following deslorelin acetate-induced puberty delay[J].Reproduction in Domestic Animals,2017,52(6):1104-1112.
[13] MAGGI R,CARIBONI A M,MARELLI M M,et al.GnRH and GnRH receptors in the pathophysiology of the human female reproductive system[J].Human Reproduction Upadte,2016,22(3):358-381.
[14] AMOSS M,BURGUS R,BLACKWELL R,et al.Purification,amino acid composition and N-terminus of the hypothalamic luteinizing hormone releasing factor (LRF) of ovine origin[J].Biochemical and Biophysical Research Communications,1971,44(1):205-210.
[15] BABA Y,MATSUO H,SCHALLY A V.Structure of the porcine LH-and FSH-releasing hormone.Ⅱ.Confirmation of the proposed structure by conventional sequential analyses[J].Biochemical and Biophysical Research Communications,1971,44(2):459-463.
[16] 金元昌,方万,李景鹏.促性腺激素释放激素的研究进展[J].黑龙江畜牧兽医,2003,3:44-45. JIN Y C,FANG W,LI J P.Progress on gonadotropin-releasing hormone[J].Heilongjiang Animal Science and Veterinary Medicine,2003,3:44-45.(in Chinese)
[17] 曹静,陈耀星,王子旭,等.促性腺激素释放激素的研究进展[J].中国畜牧兽医,2007,34(5):48-51. CAO J,CHEN Y X,WANG Z X,et al.Progress on gonadotropin-releasing hormone[J].China Animal Husbandry & Veterinary Medicine,2007,34(5):48-51.(in Chinese)
[18] 林浩然.促性腺激素释放激素(GnRH)结构与功能及其受体的进化发展[J].中山大学学报(自然科学版),2004,43(6):1-5. LIN H R.Evolutiongry aspects of the structure and functions of gonadotropin-releasing hormone (GnRH) and its receptors[J].Acta Scientiarum Naturalium Universitate Sunyatseni,2004,43(6):1-5.(in Chinese)
[19] THOMAS E J,JENKINS J,LENTON E A,et al.Endocrine effects of goserelin,a new depot luteinising hormone releasing hormone agonist[J].British Medical Journal (Clinical Research Edition.),1986,293(6559):1407-1408.
[20] BESSER G M,MCNEILLY A S,ANDERSON D C,et al.Hormonal responses to synthetic luteinizing hormone and follicle stimulating hormone-releasing hormone in man[J].British Medical Journal,1972,3(5821):267-271.
[21] 王一涵,田秀娥,江中良,等.GnRH及其类似物在动物繁殖中的应用概述[J].中国畜牧杂志,2013,49(3):89-92. WANG Y H,TIAN X E,JIANG Z L,et al.Application of GnRH and its analogues to animal reproduction[J].Chinese Journal of Animal Science,2013,49(3):89-92.(in Chinese)
[22] ROCH G J,BUSBY E R,SHERWOOD N M.GnRH receptors and peptides:Skating backward[J].General and Comparative Endocrinology,2014,209(2014):118-134.
[23] FLANAGAN C A,MANILALL A.Gonadotropin-releasing hormone (GnRH) receptor structure and GnRH binding[J].Frontiers in Endocrinology,2017,10(8):274-288.
[24] ZANDAWALA M,TIAN S,ELPHICK M R.The evolution and nomenclature of GnRH-type and corazonin-type neuropeptide signaling systems[J].General and Comparative Endocrinology,2018,264:64-77.
[25] 吕慎金,刘兆斌.促性腺激素释放激素(GnRHs)及其Ⅰ型受体在哺乳动物中的研究进展[J].中国农业大学学报,2013,18(4):147-154. LYU S J,LIU Z B.Research development of gonadotropin releasing hormone (GnRHs) and its receptor Ⅰ in the mammals[J].Journal of China Agricultural University,2013,18(4):147-154.(in Chinese)
[26] THOMPSON I R,KAISER U B.GnRH pulse frequency-dependent differential regulation of LH and FSH gene expression[J].Molecular and Cellular Endocrinology,2014,385(1-2):28-35.
[27] PINCAS H,CHOI S G,WANG Q,et al.Outside the box signaling:Secreted factors modulate GnRH receptor-mediated gonadotropin regulation[J].Molecular and Cellular Endocrinology,2014,385(1-2):56-61.
[28] MIJIDDORJ T,KANASAKI H,SUKHBAATAR U,et al.Mutual regulation by GnRH and kisspeptin of their receptor expression and its impact on the gene expression of gonadotropin subunits[J].General and Comparative Endocrinology,2017,246:382-389.
[29] 吴俊辉,曹丁壬,刘艳婷,等.家畜定时输精技术研究进展与应用现状[J].中国畜牧兽医,2016,43(7):1826-1833. WU J H,CAO D R,LIU Y T,et al.Research advances and application status of timed artificial insemination (TAI) in farm animals[J].China Animal Husbandry & Veterinary Medicine,2016,43(7):1826-1833.(in Chinese)
[30] 姜德相,董桂红,王达荣.GnRH对提高母猪受胎率和产仔存活数作用的研究[J].中国畜牧兽医,2005,32(6):28-29. JIANG D X,DONG G H,WANG D R.Effects of GnRH on improving the conception rate of sows and the number of litter size[J].China Animal Husbandry & Veterinary Medicine,2005,32(6):28-29.(in Chinese)
[31] GEISRET R D,ROSS J W,ASHWORTH M D,et al.Maternal recognition of pregnancy signal or endocrine disruptor:The two faces of oestrogen during establishment of pregnancy in the pig[J].Society of Reproduction and Fertility Supplement,2006,62(6):131-145.
[32] 吴宝云,李贺,张衡,等.不同定时输精程序对后备母猪繁殖性能的影响[J].中国畜牧杂志,2018,54(7):55-58. WU B Y,LI H,ZHANG H,et al.Effects of different fixed-time artificial insemination programs on the reproductive performance of gilts[J].Chinese Journal of Animal Science,2018,54(7):55-58.(in Chinese)
[33] CASPER R F.Basic understanding of gonadotropin-releasing hormone-agonist triggering[J].Fertility and Sterility,2015,103(4):867-869.
[34] ZILBERBERG E,HAAS J,DAR S,et al.Co-administraation of GnRH-agonist and hCG,for final oocyte maturation(double trigger),in patients with low proportion of mature oocytes[J].Gynecological Endocrinology,2015,31(2):145-147.
[35] KIRKWOOD R N,KAUFFOLD J.Advances in breeding management and use of ovulation induction for fixed-time AI[J].Reproduction in Domestic Animals,2015,50(2):85-89.
[36] VANGROENWEGHE F,GOOSSENS L,JOURQUIN J.An evaluation of gonadotropin-releasing hormone analogue administered to gilts and sows on subsequent reproductive performance and piglet birth weight[J].Porcine Health Management,2016,2:1.
[37] KAUFFOLD J,BECKJUNKER J,KANORA A,et al.Synchronization of estrus and ovulation in sows not conceiving in a scheduled fixed-time insemination program[J]. Animal Reproduction Science,2007,97(7):84-93.
[38] KHRISTI V,CHAKRAVARTHI V P,SINGH P H,et al.ESR2 regulates granulosa cell genes essential for follicle maturation and ovulation[J].Molecular and Cellular Endocrinology, 2018,474:214-226.
[39] 刘乙,马力鹏,李涛,等.ESR、OPN和RBP4基因多态性对大白猪繁殖性状的影响[J].中国畜牧兽医,2017,44(1):123-130. LIU Y,MA L P,LI T,et al.Influence of ESR,OPN and RBP4 genes polymorphisms on reproductive traits of Yorkshire sows[J].China Animal Husbandry & Veterinary Medicine,2017,44(1):123-130.(in Chinese)
[40] CHASON R J,KANG J H,GERKOWICZ S A,et al.GnRH agonist reduces estrogen receptor dimerization in GT1-7 Cells:Evidence for cross-talk between membrane-initiated estrogen and GnRH signaling[J].Molecular and Cellular Endocrinology,2015,404:67-74.
[41] KNOX R V,WILLENBURG K L,RODRIGUEZ-ZAS S L,et al.Synchronization of ovulation and fertility in weaned sows treated with intravaginal triptorelin is influenced by timing of administration and follicle size[J].Theriogenology,2011,75(2):308-319.
[42] 冯挺财,陈欠林.缩宫素、促黄体素释放激素A3对母猪繁殖性能的影响[J].中国畜牧兽医文摘,2017,33(11):71-72. FENG T C,CHEN Q L.Effect of oxytocin and LHRH-A3 on reproductive performance of sows[J].Chinese Abstracts of Animal Husbandry & Veterinary Medicine,2017,33(11):71-72.(in Chinese)
[43] KNOX R V,STEWART K R,FLOWERS W L,et al.Design and biological effects of a vaginally administered gel containing the GnRH agonist,triptorelin,for synchronizing ovulation in swine[J].Theriogenology,2018,112:44-52.
[44] ULGUIM R R,BORTOLOZZO F P,WENTZ I,et al.Ovulation and fertility responses for sows receiving once daily boar exposure after weaning and OvuGel^® followed by a single fixed time post cervical artificial insemination[J].Theriogenology,2018,105:27-33.
[45] RUTHERFORD A J,SUBAK-SHARPE R J,DAWSON K J,et al.Improvement of in vitro fertilisation after treatment with buserelin,an agonist of luteinising hormone releasing hormone[J].British Medical Journal,1988,296(39):1765-1768.
[46] MARUUCHI T,SUGIYAMA T,KATAOKA A,et al.Effects of a gonadotropin-releasing hormone agonist on rat ovarian adenocarcinoma cell lines in vitro and in vivo[J].Japanese Journal of Cancer Research,1998,89(9):977-984.
[47] DRIANCOURT M A,COX P,RUBION S,et al.Induction of an LH surge and ovulation by buserelin (as receptal) allows breeding of weaned sows with a single fixed-time insemination[J].Theriogenology,2013,80(4):391-399.
[48] MARTINAT-BOTTÉ F,VENTURI E,GUILLOUET P,et al.Induction and synchronization of ovulations of nulliparous and multiparous sows with an injection of gonadotropin-releasing hormone agonist (Receptal)[J].Theriogenology,2010,73(3):332-342.

促性腺激素释放激素及其类似物在母猪繁殖中应用的研究进展

Research Progress on Application of Gonadotropin-releasing Hormone and Its Analogues in Porcine Reproduction

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