CD46基因在新疆褐牛不同组织中的表达研究

doi:10.16431/j.cnki.1671-7236.2017.06.030

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (6): 1790-1795.doi: 10.16431/j.cnki.1671-7236.2017.06.030

CD46基因在新疆褐牛不同组织中的表达研究

木哈买提江·铁格斯, 张慧敏, 宋焕堂, 加尔肯, 苏战强, 冉多良, 刘建华

新疆农业大学动物医学学院, 乌鲁木齐 830052

收稿日期:2016-12-28 出版日期:2017-06-20 发布日期:2017-06-28
通讯作者: 刘建华 E-mail:liujianhua627@126.com
作者简介:木哈买提江·铁格斯(1988-),男,新疆阿勒泰人,硕士,研究方向:分子病毒学,E-mail:1078741254@qq.com
基金资助:
新疆维吾尔自治区自然科学基金面上项目（2013211A030）

Study on Expression of CD46 Gene in Different Tissues of Xinjiang Brown Cattle

Muhamaitijiang·Tiegesi, ZHANG Hui-min, SONG Huan-tang, Jarhin, SU Zhan-qiang, RAN Duo-liang, LIU Jian-hua

College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Received:2016-12-28 Online:2017-06-20 Published:2017-06-28

摘要/Abstract

摘要：

为了探究CD46基因在新疆褐牛不同组织中的表达丰度差异，初步掌握CD46基因在新疆褐牛不同组织中的表达规律，试验设计了特异性引物，应用实时荧光定量PCR技术分析新疆褐牛心脏、肝脏、脾脏、肺脏、肾脏、小肠、大肠、胃、肌肉、卵巢和乳腺中的CD46基因表达情况。结果表明，新疆褐牛CD46基因在所检测的11种组织中均有表达，不同组织部位的表达存在差异，其中肝脏CD46表达丰度最高，其表达丰度显著高于肺脏、脾脏、肾脏、胃、小肠、大肠、卵巢和乳腺（P< 0.05）。本研究对新疆褐牛各组织CD46基因的表达规律进行初步分析，为CD46基因表达规律与其生物功能研究的开展提供了参考依据。

关键词: CD46基因; 新疆褐牛; 组织; 表达丰度

Abstract:

In order to study the differences of CD46 gene expression in different tissues of Xinjiang Brown cattle and preliminarily master the expression regular pattern in different tissues, specific primers was designed in this study, and Real-time quantitative PCR technique was used to analyze the expression of CD46 gene in different tissues of Xinjiang Brown cattle like heart,liver,spleen,lung, kidney,small intestine,large intestine,stomach,muscle,ovarian and breast, respectively.The results showed that CD46 gene was expressed in all 11 samples of Xinjiang Brown cattle,and the expression in the liver was the highest, which was significantly higher than in lung,spleen,kidney, stomach,small intestine, large intestine, ovarian and breast (P< 0.05).By analyzing the results of CD46 gene expression in tissues of Xinjiang Brown cattle could provide information for studying the CD46 gene expression regulation pattern and biological function.

Key words: CD46 gene; Xinjiang Brown cattle; tissues; expression abundance

中图分类号:

Q786

木哈买提江·铁格斯, 张慧敏, 宋焕堂, 加尔肯, 苏战强, 冉多良, 刘建华. CD46基因在新疆褐牛不同组织中的表达研究[J]. 《中国畜牧兽医》, 2017, 44(6): 1790-1795.

Muhamaitijiang·Tiegesi, ZHANG Hui-min, SONG Huan-tang, Jarhin, SU Zhan-qiang, RAN Duo-liang, LIU Jian-hua. Study on Expression of CD46 Gene in Different Tissues of Xinjiang Brown Cattle[J]. , 2017, 44(6): 1790-1795.

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CD46基因在新疆褐牛不同组织中的表达研究

Study on Expression of CD46 Gene in Different Tissues of Xinjiang Brown Cattle

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