Abstract: A cDNA library for Moniezia expansa was constructed by switching mechanism at 5′ end of RNA transcript (SMART) technique using SMARTTM cDNA Library Construction Kit (Clontech). The total RNA was extracted from M.expansa using Trizol Reagent and mRNA was purified using Oligotex mRNA Kits. Single-strand cDNA was synthesized using PowerScriptTM reverse transcriptase, and double-strand cDNA was synthesized and amplified by long-distance PCR. The PCR products were digested by proteinase K. After digestion with SfiⅠ and size fractionation using SPIN-400TM columns, SMART cDNA was ligated to pBluescript II SK* vector. The ligation mixture was transformed into E.coli DH5α. The recombination rate of the library was 94.7%, and the titre was 2.52×105 PFU/mL. The size of inserts was about 0.5 to 3 kb, and the average length was 1.2 kb. 2642 ESTs from 5′-ends of the cDNA clones representing 1081 unigenes were obtained. The result displays M.expansa cDNA library is successfully constructed，and could serve as a valuable resource for screening and isolating specific genes for M.expansa.

Key words: Moniezia expansa; cDNA library; SMART