China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (2): 193-196.

• 疾病防治 • Previous Articles     Next Articles

Preparation of Sodium Penicillin Complete Antigen and Detection of the Antibody Level

DU Jun,JIA Fang,ZHANG Yu-yan,ZHOU Xue-zhang   

  1. (Key Laboratory of the Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
  • Contact: ZHOU Xue-zhang

Abstract: Glutaraldehyde method was used in this study to conjugate sodium Penicillin and bovine serum albumin (BSA) and ovalbumin (OVA) so as to synthetic complete antigen PEN-BSA and PEN-OVA. The UV scanning and SDS-PAGE gel electrophoresis were used to identify whether sodium Penicillin protein-coupled with the carrier successly. BALB/c mice were immunized，the titre of antiserum was detected by indirect ELISA. High-titer had been produced in all immunized BALB/c mice which made it possible to establish for further preparation of monoclonal antibodies and quickly immuno-assay kit of PEN residues.

Key words: sodium Penicillin; complete antigen; BSA; OVA

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