关岭牛肌球蛋白重链1基因5'侧翼启动子的克隆和生物信息学分析

doi:10.16431/j.cnki.1671-7236.2018.03.008

Abstract

Abstract:

The experiment was conducted to clone and analyze the sequence of MYH1 gene 5'-flanking region of Guanling cattle.The blood and tissues samples (longissimus dorsi,small intestine,hind leg muscles,heart,liver and adipose tissue) were collected,MYH1 gene 5'-flanking region of Guanling cattle was amplified by PCR,pUCm-T-MYH1 vector was constructed.The phylogenetic tree and promoter prediction was analysed by bioinformatics software,and the MYH1 gene expression in different tissues were compared by quantitative Real-time PCR.The results showed that 1 373 bp (-1 360 to ＋12 bp) sequence of MYH1 gene 5'-flanking region of Guanling cattle was successfully amplified by PCR.The results of bioinformatics analysis showed that there were 5 transcription start sites and multiple potential transcription factor binding sites.The highly conserved region of Guanling cattle,Rhinopithecus roxellana,Sus scrofa,Mus musculus,Pantholops hodgsonii and Equus asinus was in -400 to ＋100 bp of the upstream of transcription start sites,indicating that the -400 to ＋75 bp of the upstream of transcription start sites was the core.The results of quantitative Real-time PCR showed that the expression of MYH1 gene in longissimus dorsi and hind leg muscles were higher,and that in other tissues were very low,indicating MYH1 gene had different expression in different tissues.

Key words: Guanling cattle; MYH1 gene; clone; promoter prediction; bioinformatics analysis

CLC Number:

Q785

CHEN Wei, XU Houqiang, ZHOU Di, ZHANG Qingqing, ZHAO Huanping, WANG Yuanyuan. Cloning and Bioinformatic Analysis of MYH1 Gene 5'-flanking Region of Guanling Cattle[J]. , 2018, 45(3): 620-627.

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Cloning and Bioinformatic Analysis of MYH1 Gene 5'-flanking Region of Guanling Cattle

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