糖对牦牛卵母细胞体外成熟及其发育能力的影响

doi:10.16431/j.cnki.1671-7236.2017.01.021

Abstract

Abstract:

This study was designed to investigate the effect of different types and different concentrations of sugar on in vitro maturation(IVM) and developmental competence of yak oocytes, for being further research and optimization culture system of yak oocytes for efficient maturity yak oocytes and productivity of embryos. Immature yak oocytes were matured in vitro on culture medium with different concentrations (0,5 and 10 mmol/L) of glucose and sucrose in incubator for 24 h or 2 h pretreament with sugar and 22 h without sugar. Subsequently, then the maturation of oocytes,the cleavage rates and blastocyst formation rates after in vitro fertilization(IVF) were evaluated. The results showed that a medium with 5 and 10 mmol/L glucose IVM could significantly increase the yak oocytes maturation and cleavage (P<0.05), and the highest blastocyst formation rates in 10 mmol/L glucose group was significantly higher than 0 mmol/L glucose (P<0.05).10 mmol/L sucrose could increase significantly the nucleus maturation rates (P<0.05),and there was no significant difference of the blastocyst formation rates after IVF between 0 and 10 mmol/L sucrose (P>0.05). Furthermore, the nucleus maturation rates,IVF cleavage rates and blastocyst formation rates of yak oocytes which pretreated with 10 mmol/L glucose were the highest in these groups, and were higher than 0 mmol/L glucose (P<0.05). It manifested that the appropriate concentration of sugar could improve the quality of yak oocytes and embryos in vitro developmental competence, so it influenced in vitro development of yak oocytes indirectly.

Key words: oocyte; sugar; in vitro maturation; yak

CLC Number:

S814.6

SHI Xian, XIONG Xian-rong, LAN Dao-liang, HU Jia-jia, CAI Wen-yi, ZHANG Da-wei, LI Jian. Effect of Sugar on in vitro Maturation and Developmental Competence of Yak Oocytes[J]. , 2017, 44(1): 155-160.

References

[1] 殷铭阳, 周东辉, 刘建枝,等. 中国牦牛主要寄生虫病流行现状及防控策略[J].中国畜牧兽医, 2014, 41(5):227-230.
[2] Einspanier R, Schonfelder M, Muller K, et al. Expression of the vascular endothelial growth factor and its receptors and effects of VEGF during in vitro maturation of bovine cumulus-oocyte complex (COC)[J]. Mol Reprod Dev, 2002, 62(1):29-36.
[3] 贾振伟, 田见晖,安磊, 等. 牛卵母细胞体外成熟技术研究进展[J]. 中国农业科学, 2013, 46(8):1716-1724.
[4] Wang Q, Ratchfort A M, Chi M M, et al. Maternal diabetes causes mitochondrial dysfunction and meiotic defects in murine oocytes[J]. Mol Endocrinol, 2009, 23(10):1603-1612.
[5] Clark A R, Stokes Y M, Thompson J G. Estimation of glucose uptake by ovarian follicular cells[J]. Ann Biomed Eng, 2011, 39(10):2654-2667.
[6] Paczkowski M, Silva E, Schoolcarf W B, et al. Comparative importance of fatty acid beta-oxidation to nuclear maturation, gene expression, and glucose metabolism in mouse, bovine, and porcine cumulus oocyte[J].Biol Reprod, 2013, 88(5):1-11.
[7] Paczkowski M, Schoolcraftand W B, Krishe R L, et al. Fatty acid metabolism during maturation affects glucose uptake and is essential to oocyte competence[J].Reproduction,2014, 148(4):429-439.
[8] Sutton-mcdowall M L, Gilchrist R B, Thompson J G. The pivotal role of glucose metabolism in determining oocyte developmental competence[J]. Reproduction, 2010, 139(4):685-695.
[9] Silva E,Paczlowski M,Krisher R L.The effect of leptin on maturing porcine oocytesis dependent on glucose concentration[J].Mol Reprod Dev, 2012, 79(4):296-307.
[10] Hashimoto S, Minami N, Yamada M, et al. Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization:Relevance to intracellular reactive oxygen species and glutathione contents[J].Mol Reprod Dev, 2000, 56(4):520-526.
[11] Songsasen N, Wesselowski S, Carpentre J W, et al. Ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation[J]. Mol Reprod Dev, 2012, 79(3):186-196.
[12] 徐利, 戴建军, 张廷宇, 等. 红景天多糖对猪卵母细胞体外成熟及其发育潜力的影响[J]. 畜牧兽医学报, 2013, 44(4):557-561.
[13] Sato H, Iwata H, Hayashi T, et al. The effect of glucose on the progression of the nuclear maturation of pig oocytes[J]. Anim Reprod Sci, 2007, 99(3-4):299-305.
[14] 张朔, 周佳勃, 岳顺利, 等. 不同浓度葡萄糖对猪卵母细胞体外成熟及胚胎发育的影响[J]. 黑龙江畜牧兽医, 2016,2:106-108.
[15] Alvarez G M,Ferretti E L,Gutnisky C, et al. Modulation of glycolysis and the pentose phosphate pathway influences porcine oocyte in vitro maturation[J]. Reprod Domest Anim, 2013, 48(4):545-553.
[16] 霍生东, 卢建雄, 杨具田, 等. 不同浓度葡萄糖对小鼠卵母细胞体外成熟的影响[J]. 西北民族大学学报(自然科学版),2011, 32(83):61-64.
[17] Kumar P, Rajput S,Verma A, et al. Expression pattern of glucose metabolism genes in relation to development rate of buffalo(Bubalus bubalis) oocytes and in vitro produced embryos[J]. Theriogenology, 2013, 80(8):914-922.
[18] Kumar P,Verma A,Roy B,et al. Effect of varying glucose concentrations during in vitro maturation and embryo culture on efficiency of in vitro embryo production in buffalo[J]. Reprod Domest Anim, 2012, 47(2):269-273.
[19] Herrick J R, Brad A M, Krisher R L,et al. Intracellular adenosine triphosphate and glutathione concentrations in oocytes from first estrous, multi-estrous, and testosterone-treated gilts[J]. Anim Reprod Sci, 2003, 78(1-2):123-131.
[20] Downs S M, Hudson E D. Energy substrates and the completion of spontaneous meiotic maturation[J]. Zygote, 2000, 8(4):339-351.
[21] Kumar P,Verma A,Kumar M,et al. Expression pattern of glucose metabolism genes correlate with development rate of buffalo oocytes and embryos in vitro under low oxygen condition[J]. J Assist Reprod Genet, 2015, 32(3):471-478.
[22] Frank L A, Sutton-Mcdowall M, Russell D L, et al. Effect of varying glucose and glucosamine concentration in vitro on mouse oocyte maturation and developmental competence[J]. Reprod Fertil De, 2013, 25(2):1095-1104.
[23] Wang Q, Chi M M, Schedl T, et al. An intercellular pathway for glucose transport into mouse oocytes[J].Am J Physiol Endocrinol Metab, 2012, 302(12):E1511-E1518.
[24] Han Y Y,Yan J,Zhou J L, et al.Acute fasting decreases the expression of GLUT1 and glucose utilisation involved in mouse oocyte maturation and cumulus cell expansion[J]. Reprod Fertil Dev, 2012, 24(5):733-742.

Effect of Sugar on in vitro Maturation and Developmental Competence of Yak Oocytes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Rui, ZHANG Yiyang, LUO Xuehui, ZHOU Manlin, WU Shaomi, YANG Kun, QIAO Zilin. Effect of Hypoxia on Proliferation and Oxidative Stress Injury of Pulmonary Artery Smooth Muscle Cells in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2245-2254.
[2]	WU Zhijuan, CHAI Zhixin, WANG Jikun, WANG Jiabo, ZHONG Jincheng, XIN Jinwei. Expression Analysis of Specific Matrix Metalloproteinases and Functionally Related Genes of Skeletal Muscles in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1774-1784.
[3]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.
[4]	LI Mingguo, LI Qingchun, CHEN Xin, LU Shihao, HE Fan, QI Mengfan, ZHANG Huapeng, REN Yujun, ZHANG Qingze, FU Binbin, XU Mengsi, AI Zikai, YAN Kun, FENG Yun, HUA Zaidong, HUANG Tao, BI Yanzhen. Effects of TET Demethylase on Porcine Oocyte Development [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1907-1917.
[5]	TANG Yu, YANG Yifeng, ZHANG Ying, XUE Hailong, FENG Huailiang, XU Baozeng. Cytological Study on Oocyte Maturation of Raccoon Dog [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1434-1443.
[6]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[7]	WANG Yunlu, MENG Zhaoyi, YAO Yilong, GUO Min, NIU Jiaqaing, SUOLANG Sizhu, XU Yefen. Screening and Targeting Verification of lncRNA of "Sponge Adsorption" bta-miR-146a in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 859-869.
[8]	XU Xi, YANG Yuze, HAO Haisheng, DU Weihua, ZHU Huabin, YANG Baigao, ZHAO Xueming. Advances in Oocyte and Embryo Defatting Methods for Domestic Animals [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 626-637.
[9]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[10]	WEI Jia, BAI Qin, LUO Xiaolin, GUAN Jiuqiang, AN Tianwu, ZHAO Hongwen, TAN Wu, LI Huade, XIE Rongqing, SHA Quan, JIANG Mingfeng, ZHANG Xiangfei. Effects of Different Weaning Strategies on Growth, Serum Biochemical Indexes and Antioxidant Capacity of Yak Calves [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3400-3410.
[11]	BAO Qi, BAO Pengjia, MA Xiaoming, WU Xiaoyun, MENG Guangyao, CHU Min, CAO Hongmei, LANG Jianying, AN Yufeng, LIANG Chunnian, YAN Ping. Genetic Structure,Selection Signal Analysis and ROH Detection of Sunan Yak Population [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2992-3005.
[12]	MO Xianhong, YUE Kaiping, SUN Liyao, LI Bing, ZHAO Bing, GUO Cheng, XU Zhenjun. Effect of 2-APB on Development Capacity of Vitrified Bovine Mature Oocytes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2669-2676.
[13]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[14]	ZHANG Qiang, LUOSANG Dunzhu, BASANG Wangdui, PENG Yangyang, E Guangxin, NIMA Ciren, SUOLANG Duoji, BA Duo, DAN Ba, XIAN Lili, DANBA Jiacan, ZHI Zhang, PINGCUO Zhandui. Estimation of Genetic Diversity and Population Structure of Five Tibet Yak Populations Using Microsatellites Markers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2228-2238.
[15]	XIE Shuqiong, MA Shilong, TANG Jiao, LIU Yili, JIANG Mingfeng. Sequencing Analysis of Full-length Transcriptome of Abomasum in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1610-1620.