甘氨酰tRNA合成酶对奶牛乳腺上皮细胞增殖的影响

doi:10.16431/j.cnki.1671-7236.2016.06.024

Abstract

Abstract: Glycyl-tRNA synthetase is an important member of aminoacyl-tRNA synthetase family.However,the knowledge about expression and regulation mechanism of GlyRS in the development of bovine mammary gland is limited.To reveal the relationship among GlyRS and bovine mammary gland development and lactation,EdU immunofluorescence and flow cytometry were used to analyze the impact of GlyRS on the number of DNA and the influence of the cell cycle of BMEC,and the expression of Cyclin D1 of BMEC by Real-time PCR and Western blotting.The results showed that the DNA synthesis of BMEC,the numbers of cells in S and G2/M phase,and Cyclin D1 expression levels in BMEC were all increased after BMEC was transfected with a GlyRS overexpression vector,whereas all of the above indexes were reduced after BMEC was transfected with a shRNA targeting against GlyRS.This study revealed that GlyRS accelerates cell cycle transformation,increases the number of DNA synthesis by upregulating the expression of Cyclin D1,thereby promoting BMEC proliferation.

Key words: bovine mammary epithelial cells; glycyl-tRNA synthetase; cell proliferation

CLC Number:

Q813.1

GU Xin-yu, LUO Chao-chao, AO Jin-xia, HUANG Xin, ZANG Yan-li, SUN Xia, GAO Xue-jun. Effect of Glycyl-tRNA Synthetase on Cell Proliferation of Bovine Mammary Epithelial Cells[J]. , 2016, 43(6): 1557-1565.

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Effect of Glycyl-tRNA Synthetase on Cell Proliferation of Bovine Mammary Epithelial Cells

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