马流产沙门氏菌新疆分离株FliC基因的克隆及分子特征分析

Abstract

Abstract: To get the molecular biology character of Salmonella abortus equi isolated from Xinjiang and investigate the structure and function of FliC gene, we amplified FliC gene using PCR method, cloned, sequenced and analyzed FliC gene using bioinformatic methods. The results indicated that FliC genes of 2 isolates were 626 bp (GenBank: KJ486797, KJ486798). Sequence homology analysis showed that compared with those strains obtained from GenBank, the nucleotide sequence identity of the FliC gene of these isolates in Xinjiang were 49.6% to 100%. The results showed that FliC gene was conserved during their evolution. This result would provide foundation for the analysis of molecular epidemiology of this disease and the prevention of this infection using FliC gene.

Key words: Salmonella abortus equi; FliC gene; clone; molecular character analysis

CLC Number:

LI Yang, YANG Kang, ZHANG Bao-jiang, SU Yan. Cloning and Molecular Character Analysis of FliC Gene of Salmonella abortus equi Isolated from Xinjiang[J]. , 2014, 41(11): 20-24.

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Cloning and Molecular Character Analysis of FliC Gene of Salmonella abortus equi Isolated from Xinjiang

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