抗水牛伊氏锥虫变异表面糖蛋白抗原单克隆抗体的研制

›› 2009, Vol. 36 ›› Issue (10): 72-74.

• 生物技术 • Previous Articles Next Articles

Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi

ZENG Xiao-fei, CHEN Han-zhong, WEI Ying-yi, HE Mu-rong, LI Xiao-xu

(College of Animal Science and Technology, Guangxi University, Nanning 530005, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20
Contact: CHEN Han-zhong

Abstract

Abstract: The BALB/c mice were immunized with variant surface glucoprotein of Trypanosoma evansi. The spleen lymph cells were fused with mouse SP2/0 myeloma cells, two hybridoma cell lines 3D7 and 5B9 secreting monoclonal antibodies against variant surface glucoprotein were determined by indirect ELISA. Their titers in cell culture supernatant were 1∶6400 and 1∶12800, and titers of ascites were 1∶105 and 1∶106, both 3D7 and 5B9 belongs to IgG1 with κ chain.

Key words: variant surface glucoprotein; Trypanosoma evansi; monoclonal antibody

CLC Number:

S859.79

ZENG Xiao-fei;CHEN Han-zhong;WEI Ying-yi;HE Mu-rong;LI Xiao-xu. Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi[J]. , 2009, 36(10): 72-74.

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Preparation of Monoclonal Antibody against Variant Surface Glucoprotein Antigen of Trypanosoma evansi

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