犬瘟热—犬冠状病毒双重PCR诊断方法的建立及初步应用

Abstract

Abstract: Two pairs of PCR primers were designed according to sequence of canine distemper virus （CDV） and canine coronavirus （CCV） from GenBank, respectively, which could amplify 550 bp fragment for CDV and 225 bp fragment for CCV. The products of PCR were cloned to pMD18-T for sequencing, which proved to be specific. Positive plasma were developed for standard DNA, and the sensitivity result of duplex PCR showed that the method could amplify 0.1 ng/μL nucleic acid for both of viruses. The result of rudimentary application showed that the method was specific, sensitive, efficient, and was a new detecting method for the mixed infection of CDV and CCV.

Key words: canine distemper virus; canine coronavirus; duplex PCR

SUN Yuan-yuan, ZHAO Peng, LIU Jun, WANG Yun, QIAN Ke. Development and Application of Duplex PCR for Canine Distemper Virus and Canine Coronavirus Detection[J]. .

References

1 刘大飞, 姜一曈, 杨天阔, 等. 同时检测犬瘟热病毒和犬细小病毒双重PCR方法的建立［J］. 中国预防兽医学报, 2012, 34(4): 211～213.
2 孙园园, 宫文妮, 黄娟, 等. 犬瘟热病毒H基因克隆测序与原核表达［J］. 中国畜牧兽医, 2009, 36(5): 58～62.
3 李天松, 王磊, 王胜乐, 等. 犬瘟热病毒检测方法研究进展［J］. 中国病原生物学杂志, 2012, 7(4): 305～308.
4 柳强, 侯喜林, 车车, 等. 牛冠状病毒和轮状病毒的双重PCR检测方法的建立及应用［J］. 中国预防兽医学报, 2009, 31(9): 701～704.
5 耿庆华, 林颖, 裴程程, 等. 犬瘟热病毒和犬腺病毒2型双重PCR方法的建立及应用［J］. 中国动物检疫, 2012, 29(5): 38～41.
6 程悦宁, 易立, 司方方, 等. 我国毛皮动物主要传染病防控现状及防控建议［J］. 经济动物学报, 2013, 17(1): 49～54.
7 董炳梅, 唐娜, 王金良, 等. 胞内劳森氏菌PCR检测方法的建立与应用［J］. 中国预防兽医学报, 2011, 33(5): 370～373.
8 Cha S Y, Kim E J, Kang M, et al. Epidemiology of canine distemper virus in wild raccoon dogs (Nyctereutes procyonoides) from South Korea［J］. Comparative Immunology, Microbiology and Infectious Diseases, 2012, 35(5): 497～504.
9 Erles K, Toomey C, Brooks H W, et al. Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease［J］. Virology, 2003, 310(2): 216～223.
10 Mitchell J A, Brooks H, Shiu K B, et al. Development of a quantitative real-time PCR for the detection of canine respiratory coronavirus［J］. Journal of Virological Methods, 2009, 155(2): 136～142.
11 Soma T, Ohinata T, Ishii H, et al. Detection and genotyping of canine coronavirus RNA in diarrheic dogs in Japan［J］. Research in Veterinary Science, 2011, 90(2): 205～207.
12 Yi L, Cheng S, Xu H, et al. Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs［J］. Journal of Virological Methods, 2012, 179(1): 281～287.

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Development and Application of Duplex PCR for Canine Distemper Virus and Canine Coronavirus Detection

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