牛血Cu/Zn-SOD提取条件的研究

Abstract

Abstract: In this assay, bovine blood as a sample, was studied the effect of four factors that concentrations of Cu²+ and Zn²+, denaturation temperature and thermal time on the activity of Cu/Zn-SOD in bovine blood. Four factors and five levels of orthogonal experiment was designed, and combined with methods of pyrogallol to measure the activity of enzyme, Coomassie brilliant blue method to determine the content of Cu/Zn-SOD protein, and polyacrylamide gel electrophoresis to detect the purity of the protein. We finally found the optimum extraction conditions of Cu/Zn-SOD in bovine blood. The results showed that when the concentrations of Cu²+ and Zn²+ were 6 and 12 mmol/L,respectively,at 90 ℃ thermal denaturation with 35 min, we could get Cu/Zn-SOD which had a higher activity. This study was aimed to provide a convenient and economical method for Cu/Zn-SOD extraction in bovine blood.

Key words: bovine blood; Cu/Zn-SOD; extraction conditions; activity

LIU Jin，ZHANG Feng，WEI Jian-min. Study on Extraction Conditions of Cu/Zn-SOD for Bovine Serum[J]. China Animal Husbandry & Veterinary Medicine.

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Study on Extraction Conditions of Cu/Zn-SOD for Bovine Serum

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