大鼠原代肝脏细胞的分离培养及其膜受体鉴定

Abstract

Abstract: This research adopted improved collagenase perfusion isolation to isolate and culture rat hepatocytes, and had carried on the observation of morphology, identification of function and membrane receptor to explore the optimal state of its short-term cultures. The results showed that 2.12 ×10⁸ hepatocytes per rat liver were obtained,and the average motility rate of cells was 94.23%. Hepatocytes were polygonal and grew into flakinesst under light microscope. We found that hepatocytes culture in vitro were better on the third day by the continual detection of LDH, Alb and urea. Function of growth hormone receptor on liver membrane had not been damaged by the identification of Mab263-PE, which laid the foundation for the cell signal transduction and relevant test.

Key words: rats; hepatocyte; primary culture; growth hormone receptor; identification

CLC Number:

Q813.1⁺1

LIU Yu, LAN Hai-nan, LI Wei, YANG Yan-hong, FU Zhi-ling, MA Si-hui, WU Tian-cheng, GUO Feng, ZHAO Xue, ZHANG Hui, CUI Huan-zhong, WU Min, YANG Huan, LI Yu-meng, HONG Pan, ZHENG Xin. Isolation and Culture of Rat Primary Hepatocytes, and Identification of its Membrane Receptor[J]. , 2013, 40(10): 144-147.

References

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Isolation and Culture of Rat Primary Hepatocytes, and Identification of its Membrane Receptor

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