›› 2013, Vol. 40 ›› Issue (7): 167-171.

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Isolation,Identification and Drug Sensitive Assay of Salmonella From Broiler

WANG Ao-xue1,2, ZHANG Ling-yun2, ZHANG An-chao3, LI Kun-peng2,4, ZHANG Xiu-li2, LIANG Wan-feng1, ZHANG Lin-bo2   

  1. 1. Yanbian University, Yanji 133000, China;
    2. Jilin Agricultural University, Changchun 130118, China;
    3. Jilin China Tai Enterprise Co., Ltd., Changchun 130033, China;
    4. Institute of Animal Sciences, China Academy of Agricutural Sciences, Beijing 100193, China
  • Received:2012-12-25 Online:2013-07-20 Published:2013-07-24

Abstract: To find out the situations about infection of Salmonella and its drug resistance somewhere in Jilin province,the bacteria were isolated from 150 tissue samples of sick broiler of 7 farms in this city,and hemagglutinin test, pathogenity test and drug sensitive test were carried out. 26 strains were isolated, and 19 strains were identified positive by the HA,of which five were strongly positive and killed broiler, drug sensitive test results showed that the 5 strains of Salmonella were more sensitive to cefotaxime, and very high resistance to ampicillin,tilmicosin,lincomycin,azithromycin and cephradine,resistance rates as high as 100%. Norfloxacin,enrofloxacin,ciprofloxacin,doxycycline and chloramphenicol didn’t have synergistic or additive effect with mequindox which was used in clinical. These results suggested that Salmonella was one of the main pathogens harmful to the broiler of farms, and resistance was very serious.

Key words: Salmonella; isolation and identification; drug sensitive test

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