王不留行对奶牛乳蛋白合成信号转导通路的影响

Abstract

Abstract: To study the effect of Vaccaria segetalis on cellular signal transduction pathway associated with milk protein synthesis in dairy cow,water extraction of Vaccaria segetalis was prepared.Western blotting was used to study the changes of the signal molecules associated with milk protein synthesis of dairy cow mammary gland epithelial cells (DCMECs), treated with estrogen (ER), prolactin (PRL) and Vaccaria segetalis. The results showed that the Vaccaria segetalis extraction liquid, ER and PRL could promote milk protein synthesis and up-regulate the expression of p-STAT5,p100,GAS,S6K1 and p-mTOR, furthmore, methionine could enhance this action. These datas suggested that Vaccaria segetalis had a similar role with ER/PRL and up-regulated expression of the genes of DCMECs for lactation on the translational and transcriptional level.

Key words: Vaccaria segetalis; methionine; estrogen; prolactin; dairy cow; signal transduction pathway; milk protein

CLC Number:

S823.5

MENG Hai-yang, SUN Xiao-xu, WANG Li-na, LIN Ye, YUAN Xiao-han, LI Qing-zhang, GAO Xue-jun. Effect of Vaccaria segetalis on Cellular Signal Transduction Pathway Associated with Milk Protein Synthesis in Dairy Cow[J]. , 2013, 40(4): 121-124.

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Effect of Vaccaria segetalis on Cellular Signal Transduction Pathway Associated with Milk Protein Synthesis in Dairy Cow

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