悬浮培养Sf21细胞高效表达牛γ-干扰素及其特性鉴定

Abstract

Abstract: In the study, we mainly did the research about efficient expression and characterization of bovine interferon-γ in suspension Sf21 cells. Bovine IFN-γ gene was amplified by RT-PCR, and inserted into the pFastBac^TM HTA to get the recombinant plasmid, named as pFastBac^TMHTA-IFN-γ. After transformation and transfection,the recombinant baculovirus was obtained. The target protein named rBovIFN-γ was successfully expressed in Sf21 insect cells during suspension culture. We identified the rBovIFN-γ with SDS-PAGE,Western blotting and mass spectrum(MS). Western blotting analysis showed that the recombinant protein had good activity. The protein MS identification results confirmed the recombinant bovine IFN-γ expression via the baculovirus expression system. MDBK/VSV antiviral test results showed that the rBovIFN-γ had high antiviral activity at 1.05×10⁵ IU/mg.

Key words: bovine interferon-γ; baculovirus; suspension culture; MS; antiviral

CLC Number:

WANG Hai-chun, JIA Hong, YUAN Wei-feng, HOU Shao-hua, GUO Xiao-yu, XIN Ting, ZHU Hong-fei. Efficient Expression and Characterization of Bovine Interferon-γ in Suspension Sf21 Cells[J]. , 2012, 39(11): 1-6.

References

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Efficient Expression and Characterization of Bovine Interferon-γ in Suspension Sf21 Cells

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