喹诺酮类耐药决定区、外排泵负调控基因对大肠杆菌氟喹诺酮高水平耐药分子机制的影响

Abstract

Abstract: The objective of this study was to detect the role of QRDR, acrR, marR and soxR in genetic characterization of highly fluoroquinolone-resistant clinical Escherichia coli strains. PCR method was used to analyse the mutations of QRDR, acrR, marR and soxR. RT-PCR method was used to detect the expression level of efflux pump and porin related genes. Mutations of QRDR mainly fell into GyrA(Ser83Leu and Asp87Asn) and ParC(Ser80Ile), and other mutations had also detected, such as ParC Glu84Gly, Glu84Lys, Glu84Val and Glu84Ala,ParE Ser458Ala,et al. There was an insertion of 777 bp fragment within acrR in ED28. 12 of 18 stains including ATCC25922 had double mutations (Gly103Ser and Tyr137His) in MarR and two (EP26 and EG42) of them had an insertion. ED40 had amino acid substitution (Thr38Ser and Gly74Arg) in SoxR. In multiple mutations strains, there were markedly increased expression of AcrAB and decrease expression of porin or porin loss.

Key words: QRDR; acrR; marR; soxR; resistance; fluoroquinolone

CLC Number:

S852.61⁺4

REN Yan-na, ZHEN Pan-pan, LI Jian, GUO Yu-fang, WANG Li-hua, REN Jun-yu, ZHANG Wen-hui, JIANG Hong-xia. Genetic Characterization of Highly Fluoroquinolone Resistant Clinical Escherichia coli Strains:Role of QRDR,acrR,marR and soxR[J]. , 2012, 39(10): 11-16.

References

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Genetic Characterization of Highly Fluoroquinolone Resistant Clinical Escherichia coli Strains:Role of QRDR,acrR,marR and soxR

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