合作猪TRIF基因CDS区克隆鉴定及组织表达分析

doi:10.16431/j.cnki.1671-7236.2025.04.003

Abstract

Abstract: 【Objective】 TIR domain-containing adaptor protein inducing interferon β (TRIF) gene played the important roles in host resistance and immunity.This study was aimed to study the sequence characteristics and tissue expression of CDS region of TRIF gene in Hezuo pigs.【Method】 Gansu Hezuo pigs was taken as the research object,the full-length CDS region sequence of TRIF gene was cloned and subjected to bioinformatics analysis.The expression levels of TRIF gene were detected from 8 tissues including lung,kidney,spleen,longissimus dorsi muscle,cecum,ileum,rectum and colon through Real-time quantitative PCR.【Result】 The full length of TRIF gene CDS region was 2 142 bp and encoded 713 amino acids,which was 99.81% similarity to TRIF gene sequence of Sus scrofa in NCBI database (accession No.:NM_001315738.2).Phylogenetic tree results showed that Hezuo pigs were closest to Sus scrofa and furthest to Danio rerio.TRIF protein of Hezuo pigs had hydrophilicity,no transmembrane region,no signal peptide and no N-glycosylation modification sites,which was mainly located in the nucleus.TRIF protein was mainly composed by random coils of secondary structure.The tertiary structure prediction was basically consistent with the secondary structure.The protein had six low-complexity domains and a winding coil region.Real-time quantitative PCR results showed that TRIF gene of Hezuo pigs was expressed in eight tissues including lung,kidney,spleen,longissimus dorsi muscle,cecum,ileum,rectum and colon,with the highest expression in longissimus dorsi muscle.【Conclusion】 This study had successfully cloned the CDS region sequence of TRIF gene in Hezuo pigs.The highest expression was found in longissimus dorsi muscle.The results could provide a valuable reference for the further study of TRIF gene in Hezuo pigs.

Key words: Hezuo pigs; TRIF gene; bioinformatics; expression

CLC Number:

S828.8

HU Huihui, FU Panpan, LI Jie, YAN Zunqiang, GAO Xiaoli, YANG Jiaojiao, HUANG Xiaoyu. Cloning,Identification and Tissue Expression Analysis of TRIF Gene CDS Region in Hezuo Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1478-1487.

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Cloning,Identification and Tissue Expression Analysis of TRIF Gene CDS Region in Hezuo Pigs

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