苦苣菜对肺炎小鼠炎症因子及HMGB1/TLR4/NF-κB信号通路的影响

doi:10.16431/j.cnki.1671-7236.2023.12.044

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the effects of Sonchus oleraceus L.aqueous extract on inflammation-related factors and inflammatory signaling pathways in mouse pneumonia model induced by lipopolysaccharide (LPS),to explore the anti-inflammatory effects of Sonchus oleraceus L.aqueous extract on mouse pneumonia.【Method】 Preparation of aqueous extract of Sonchus oleraceus L.,the test mice were randomly divided into blank group,model group,positive control group,and Sonchus oleraceus L.aqueous extract low,medium and high concentrations groups,mice in blank group were not treated,mice in model group were gavaged with 0.3 mL of saline,mice in positive control group were gavaged with 0.3 mL of 5 mg/kg dexamethasone,and mice in Sonchus oleraceus L.aqueous extract low,medium and high concentrations groups were gavaged with 0.3 mL of 100,200 and 400 mg/mL Sonchus oleraceus L.aqueous extract,respectively.After being administered by gavage for 1 week,mice in blank group were injected intraperitoneally with 0.3 mL of saline,and mice in the rest of groups were injected intraperitoneally with 0.3 mL of 30 mg/kg LPS to induce the establishment of a mouse pneumonia model. The levels of interleukin-6 (IL-6),IL-10 and transforming growth factor-β (TGF-β) in serum of each group were measured by ELISA,and the expression levels of inflammatory signaling pathway (HMGB1/TLR4/NF-κB) proteins in lungs of mice in each group were measured by IHC and Western blotting.【Result】 Compared with blank group,the serum levels of IL-6 in mice were significantly up-regulated (P＜0.05),the levels of IL-10 and TGF-β in mice were up-regulated,and the distribution and expression of HMGB1,TLR4 and NF-κB proteins in lung of mice were highly significantly up-regulated in model group (P＜0.01).Compared with model group,the serum levels of pro-inflammatory factor IL-6 in mice was down-regulated,anti-inflammatory factor IL-10 was up-regulated,and anti-inflammatory factor TGF-β was highly significantly up-regulated in Sonchus oleraceus L.aqueous extract-treated groups (P＜0.01).HMGB1,TLR4 and NF-κB was abundantly distributed on the epithelial cells surrounding the alveoli in lungs.Compared with model group,the distribution of HMGB1,TLR4 and NF-κB were highly significantly down-regulated in lung of mice treated with aqueous extract of Sonchus oleraceus L.(P＜0.01),and showed a dose-dependent effect,the protein expression of HMGB1 and TLR4 were exremely significantly or down-regulation (P＜0.01),and the protein expression of NF-κB was significantly or extremely significantly down-regulated (P＜0.05 or P＜0.01).【Conclusion】 The aqueous extract of Sonchus oleraceus L.could inhibit the secretion of pro-inflammatory factor IL-6 and the expression of signaling pathway HMGB1,TLR4 and NF-κB in lung of mice with pneumonia induced by intraperitoneal injection of LPS,and promote the secretion of anti-inflammatory factor IL-10 and TGF-β in serum of mice,indicating that the aqueous extract of Sonchus oleraceus L.had anti-pneumonia effect.

Key words: Sonchus oleraceus L.; pneumonia; inflammatory factors; HMGB1/TLR4/NF-κB

CLC Number:

S852.21

LI Mengyang, CHAO Bing, ZHOU Tao, ZHANG Jianping. Effect of Sonchus oleraceus L.on Inflammatory Factors and HMGB1/TLR4/NF-κB Signaling Pathway in Mice with Pneumonia[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5232-5242.

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Effect of Sonchus oleraceus L.on Inflammatory Factors and HMGB1/TLR4/NF-κB Signaling Pathway in Mice with Pneumonia

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