1株唾液乳杆菌的抑菌物质及益生作用

doi:10.16431/j.cnki.1671-7236.2023.09.033

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the bacteriostatic substances and probiotics of Lactobacillus salivarius from chicken,and to explore the possibility of its application in production.【Method】 In this experiment,sell free supernatant (CFS) was prepared by vacuum freezing Lactobacillus salivarius fermentation broth.The antibacterial effect of CFS was observed by Oxford cup method,and the antibacterial substances were analyzed by changing pH,adding catalase and various proteases.Twenty mice were randomly divided into control group and Lactobacillus salivarius group to study the probiotic effects of Lactobacillus salivarius.【Result】 CFS had obvious inhibitory effect on Escherichia coli and Staphylococcus aureus in vitro.When the pH of CSF was 5.0,the antibacterial activity disappeared.Except pepsin,the antibacterial activity of CSF treated with catalase and other proteases decreased significantly compared with untreated CSF (P＜0.05 or P＜0.01),indicating that the antibacterial substances contained organic acids,hydrogen peroxide and protein substances.The survival rate of mice in Lactobacillus salivarius group was 100%,duodenal villus morphology was significantly better than that of control group,liver index,spleen index and jejunum Occludin mRNA expression had no signifcant difference with control group (P＞0.05).Body weight and expression of Claudin-2 mRNA in jejunum were significantly higher than those in control group (P＜0.01).【Conclusion】 Lactobacillus salivarius had strong bacteriostatic ability in vitro and had good probiotic effect on mice.The bacteriostatic substances included organic acids,hydrogen peroxide and protein substances,which had the potential to be used as animal microecological preparations.

Key words: Lactobacillus salivary; antimicrobial substances; organ index; Occludin; Claudin-2

CLC Number:

S831.5

LI Ping, LI Tao, QI Meng, GUO Xuefeng, WU Zihao, CHE Liyan, GUO Jiadong, CHEN Rong. Bacteriostatic Substances and Probiotics of a Strain of Lactobacillus salivarius[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3771-3780.

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Bacteriostatic Substances and Probiotics of a Strain of Lactobacillus salivarius

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