猪多潜能干细胞系的研究进展

doi:10.16431/j.cnki.1671-7236.2023.01.007

Abstract

Abstract: Pig pluripotent stem cells are a kind of stem cells established in vitro with the capability of self-renewal and three germ layers differentiation, which can be used in developmental biology research, genome editing and disease modeling establishment.It has great value of application in animal husbandry production and regenerative medicine research.The culture system is of great significance for the successful construction of pluripotent stem cells, which contains a variety of components, including basic culture medium, amino acids and other nutrients, small molecular compounds (cytokines and signal pathway activators or inhibitors) to maintain cell pluripotency and inhibit its differentiation.There are many previous reports about porcine pluripotent stem cells, but effective culture system has not been obtained to maintain the long-term passage of porcine pluripotent stem cells and complete germline chimerism.Porcine pluripotent stem cells can be divided into three pluripotent states:Naïve state, Formative state and Primed state.And they can be classified into porcine expanded pluripotent stem cells, porcine embryonic stem cells, porcine pregastrulation stem cells and porcine induced pluripotent stem cells according to the derivation of the cell lines.Overall, the authors summarized the functional influences of current application of cytokines and signal pathway activators or inhibitors on the pluripotency and differentiation ability of porcine pluripotent stem cells.The present review could provide guidlines for the establishment of Naïve pluripotency porcine pluripotent stem cells with germline chimerism.

Key words: porcine; pluripotent stem cell lines; pluripotency

CLC Number:

S828

WANG Zhilong, LIU Li, ZHANG Lu, GAO Shuai. Research Progress of Porcine Pluripotent Stem Cell Lines[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 67-75.

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Research Progress of Porcine Pluripotent Stem Cell Lines

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