绵羊MyoG基因内含子Ⅱ多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.04.018

Abstract

Abstract: 【Objective】 This study was aimed to analyze the polymorphism of myogenin (MyoG) gene intron Ⅱ and its effect on growth traits in sheep,the molecular markers with significant effects on the growth and development of sheep were screened to provide reference for molecular breeding in sheep.【Method】 Five species of Large-tailed Han sheep,Small-tailed Han sheep,Yuxi Fat-tailed sheep,Dorper sheep and Hu sheep were selected as the research object. The intron Ⅱ of MyoG gene (Eco72 Ⅰ) was genotyped by PCR-RFLP technology.The population genetic polymorphism of MyoG gene intron Ⅱ was calculated by PopGene32 software.The association analysis between the different genotypes of MyoG gene intron Ⅱ and growth traits of sheep was performed by SPSS 17.0 software.【Result】 There were three genotypes of AA (368/540 bp),AB (908/368/540 bp) and BB (908 bp) were found of MyoG gene intron Ⅱ in Small-tailed Han sheep,Dorper sheep and Hu sheep populations,and there were two genotypes of AB (908/368/540 bp) and BB (908 bp) were found in Large-tailed Han sheep and Yuxi Fat-tailed sheep populations.The genotype frequency of AB in Large-tailed Han sheep,Small-tailed Han sheep,Yuxi Fat-tailed sheep,Hu sheep and Dorper sheep were 0.845,0.633,0.917,0.706 and 0.811,the genotype frequency of BB were 0.155,0.033,0.083,0.176 and 0.054,and the genotype frequency of AA genotype were 0,0.333,0,0.118 and 0.135,respectively.The lowest heterozygosity was 0.455 in Small-tailed Han sheep,and the highest was 0.497 in Dorper sheep,indicating that Dorper sheep had a higher genetic diversity than other population.The polymorphic information content (PIC) of five sheep population was moderate polymorphism (0.25<PIC<0.5).The association analysis results showed that the chest circumference,chest width,head length and neck length of BB genotype of MyoG gene intron Ⅱ in Small-tailed Han sheep were significantly lower than those of AA and AB genotypes (P<0.05).【Conclusion】 The Eco72 Ⅰ site of MyoG gene intron Ⅱ could be used as a molecular marker affecting the growth trait in sheep,and the results provided reference for molecular marker assisted selection of growth trait in sheep in the future.

Key words: sheep; MyoG gene; intron Ⅱ; Eco72 Ⅰ site; growth traits

CLC Number:

S813.3

LI Jingyun, WANG Xinle, ZHANG Yunfei, BAI Junyan, LI Gan, LEI Ying, DONG Zhihao, CHEN Yu, FAN Hongdeng, WANG Longwei, CHEN Mengke, WANG Xingke, SIMA Leiyang, ZHANG Shiwen. Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1364-1373.

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Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep

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