卵巢肿瘤去泛素化酶7A基因与鹅肥肝形成关系的研究

doi:10.16431/j.cnki.1671-7236.2022.04.011

Abstract

Abstract: 【Objective】 The experiment was aimed to study the relationship between the ovaries tumor deubiquitinase 7A (OTUD7A) gene and the formation of goose fatty liver.【Method】 40 70-day-old male Landes geese with similar body weight were selected and randomly divided into two groups.The geese of control group were free to feed,and the geese of experimental group were overfeeding.The daily intake was 500 g for the 1st to 5th day of filling,800 g for the 6th to 12th day,and 1 200 g for the 13th to 19th day.After overfeeding for 7,14 and 19 days,six geese in each group were randomly selected and slaughtered for liver samples.The expression levels of OTUD7A gene in livers of different overfeeding stages were determined by Real-time quantitative PCR.The goose primary hepatocytes were isolated from Landes goose embryos after 23 days of incubation using type Ⅳ collagenase digestion method.Goose primary hepatocytes were treated with 0 (blank control group),125 and 250 mmol/L glucose,0 (blank control group),50,100 and 200 nmol/L insulin,0 (blank control group),0.125 and 0.250 mmol/L oleic acid,linoleic acid,and 0 (blank control group),0.25 and 0.50 mmol/L palmitic acid,respectively,and the effects of these factors that related to fatty liver formation on the expression level of OTUD7A gene were detected by Real-time quantitative PCR.In addition,the overexpression vector pcDNA3.1-OTUD7A was constructed,and the constructed overexpression recombinant plasmid was transfected into goose primary hepatocytes,and the differentially expressed genes were screened by transcriptomic sequencing (RNA-Seq),and the obtained differentially expressed genes were analyzed by GO function enrichment.【Result】 OTUD7A gene expression in fatty liver was significantly higher than that in control group at days 7 and 14 of overfeeding (P<0.05).Compared to blank control group,OTUD7A gene expression in goose primary hepatocytes was up-regulated by 250 mmol/L of glucose,and 0.125 or 0.250 mmol/L of palmitate (P<0.05).Transcriptomic sequencing results revealed that a total of 34 differentially expressed genes were screened after OTUD7A gene overexpression,of which 19 genes were up-regulated and 15 genes were down-regulated.The GO function enrichment analysis of RNA-Seq showed that the entries were annotated as defense response to other organism,response to external biotic stimulus,T cell differentiation,extracellular exosome and so on.The expressions of CLMP,PROCR,SH3BP1,ARHGAP28,ACE,OTUD7A,LOC106045877 and LOC106048002 genes were up-regulated,and the expressions of TNFSF8,DDX60,PLAC8,RSAD2,MX1,RSAD2 and GBP1 genes were down-regulated.【Conclusion】 The expression level of OTUD7A gene was increased during the formation of goose fatty liver,OTUD7A might participate in the development of goose fatty liver through regulating the expressions of TNFSF8,RSAD2,MX1,GBP1,CLMP and PROCR.

Key words: goose; fatty liver; OTUD7A gene; glucose; insulin; fatty acids

CLC Number:

S835

SUN Qingyun, FAN Xiang, XING Ya, ZHAO Minmeng, LIU Long, GENG Tuoyu, GONG Daoqing. Study on the Relationship Between OTUD7A Gene and Goose Fatty Liver Formation[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1295-1303.

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Study on the Relationship Between OTUD7A Gene and Goose Fatty Liver Formation

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