2018-2020年中国部分省市猪流行性腹泻病毒S1基因的监测及遗传变异分析

doi:10.16431/j.cnki.1671-7236.2022.02.003

Abstract

Abstract: 【Objective】 This study was aimed to survey the prevalence of Porcine epidemic diarrhea virus (PEDV) in large-scale pig farms in China.【Method】 2 391 samples collected from 318 large-scale pig farms in 11 provinces and municipalities of China from 2018 to 2020 were tested for PEDV nucleic acid using RT-PCR.The S1 gene of 30 positive samples were amplified and sequenced, and analyzed by MegAlign, Mega 7.0 and other softwares.【Result】 From 2018 to 2020, the PEDV nucleic acid positive rates in pig farms were 48.57%, 10.96%, and 4.72%, respectively, and the PEDV nucleic acid positive rates in samples were 28.97%, 8.27%, and 7.50%, respectively.The positive rate of PEDV nucleic acid in pig farms and samples showed a downward trend year by year.The similarity analysis of S1 gene showed that all the 30 strains obtained in this experiment were GⅡ genotype, of which 17 strains were GⅡa subtype, 5 strains were GⅡb subtype, and 8 strains were GⅡc subtype, GⅡa subtype strains were the main types of strains circulating from 2018 to 2020.The nucleotide similarity among the 30 strains sequence was 93.0%-99.5%, and the amino acid similarity was 91.7%-100%, compared with the GⅡ genotype strains prevalent from 2017 to 2018, the S1 amino acid sequences of 22 strains showed characteristic insertion and deletion.【Conclusion】 The results provided clinical data support for surveying and analyzing the variation and evolution of PEDV in China, and provided reference for porcine epidemic diarrhea prevention and control and vaccine development.

Key words: Porcine epidemic diarrhea virus(PEDV); S1 gene; genetic variation analysis

CLC Number:

S852.65⁺1

YAN Zhong, YANG Hanchun. Surveillance and Genetic Variation Analysis of S1 Gene of Porcine Epidemic Diarrhea Virus in Part Provinces and Municipalities of China from 2018 to 2020[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 424-434.

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Surveillance and Genetic Variation Analysis of S1 Gene of Porcine Epidemic Diarrhea Virus in Part Provinces and Municipalities of China from 2018 to 2020

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