山豆根多糖对猪圆环病毒Ⅱ型感染猪肺泡巨噬细胞炎性因子水平的影响

doi:10.16431/j.cnki.1671-7236.2021.05.034

Abstract

Abstract: To investigate the effect of Sophora subprosrate polysaccharide (SSP) on the levels of inflammatory factors in 3D4/2 cells infected by Porcine circovirus virus Ⅱ type (PCV2),in this study,blank control group,PCV2 group,lipopolysaccharide (LPS) group and three concentrations of SSP group (100,200 and 400 μg/mL) were established,respectively.3D4/2 cells was infected by PCV2 in vitro, and then the cells were treated with different concentrations of SSP for 24 h.The secretions of monocyte chemotactic protein 1 (MCP-1) and interleukin-8 (IL-8) and the enzyme activities of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) were detected by ELISA assays.The mRNA expression of inducible nitric oxide synthase (iNOS) and COX-2 genes were detected by Real-time quantitative PCR,and the protein expression of iNOS and COX-2 were detected by Western blotting.The results showed that the secretions of MCP-1,IL-8 and the activities of intracellular COX-1 and COX-2 were extremely significantly higher after PCV2 infection than those in the blank control group,the mRNA expression levels and protein expression of intracellular iNOS and COX-2 were also extremely significantly increased (P<0.01);After treatment with SSP at 100,200 and 400 μg/mL,the secretions of MCP-1,IL-8 and the activities of COX-1 and COX-2 in the cells were significantly or extremely significantly decreased (P<0.05,P<0.01),the effect was most significant in 400 μg/mL SSP group,and the mRNA expression of intracellular iNOS and COX-2 gene was also significantly or extremely significantly decreased(P<0.05,P<0.01).The increase of iNOS protein expression induced by PCV2 was significantly or extremely significantly inhibited by treatment with SSP at 100,200 and 400 μg/mL (P<0.05,P<0.01).The increase of COX-2 protein expression was significantly inhibited by treatment with SSP at 200 and 400 μg/mL,and the best effect was obtained at 400 μg/mL.The results showed that SSP could inhibit the increase of mRNA expression and protein expression of pro-inflammatory factors caused by PCV2 infection and played a certain role in alleviating inflammation.

Key words: Sophora subprosrate polysaccharide; PCV2; 3D4/2; inflammatory factors

CLC Number:

S859.7

JIA Nina, CAO Mixia, YANG Jian, LIU Mengqian, CHEN Qi, WEI Yingyi, HU Tingjun. Effect of Sophora subprosrate Polysaccharide on the Levels of Inflammatory Factors in 3D4/2 Cells Infected with Porcine Circovirus Virus Ⅱ Type[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1825-1831.

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Effect of Sophora subprosrate Polysaccharide on the Levels of Inflammatory Factors in 3D4/2 Cells Infected with Porcine Circovirus Virus Ⅱ Type

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