褐黄血蜱雌蜱不同消化阶段中肠蛋白组学分析

doi:10.16431/j.cnki.1671-7236.2021.02.023

Abstract

Abstract: The aim of this study was to explore the protein components of midgut contents of adult female haemaphysalis flava collected from hedgehog body surface at four different stages and reveal the types and content changes of protein involved in blood meal digestion.The midgut contents from Haemaphysalis flava at four different stages were detected by LC-MS/MS,and the peptides and proteins obtained were identified and analyzed by using the computer software Mascot 2.2 based on previous established salivary and midgut transcriptome translation library and UniProt database.The results of this study showed that a total of 3 046 unique peptides and 303 proteins were detected and identified.Total of 271 proteins with high credible among 303 proteins identified.Among 271 high credible proteins,23 proteins had abundant contents at first stage,125 types of proteins had zero contents at later stages (from second stage to fourth stage),123 types proteins' contents changed in different degrees.Among 123 proteins with contents changed,there were 24 proteins with contents obvious change,which include 12 proteins that their contents rose obviously and 12 proteins that their contents descended obviously at four stages.It was determined that 148 high credible proteins came from hedgehog serum,and in which 24 high credible proteins may be involved in ticks digestion blood meal.

Key words: LC-MS/MS; Haemaphysalis flava; midgut proteins

CLC Number:

LI Zhongbo, YAO Guomin, HOU Qianghong, CHENG Tianyin. Proteomic Profiling of the Midgut Contents of Female Haemaphysalis flava at Different Digestion Stage[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 607-618.

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Proteomic Profiling of the Midgut Contents of Female Haemaphysalis flava at Different Digestion Stage

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