鸡精液稀释配方对低温保存精子活力和输精效果的影响

doi:10.16431/j.cnki.1671-7236.2020.06.023

Abstract

Abstract: For the improvement of the skills of semen preservation and the usage of semen of chicken farms in Southern China,the effects of different storage time (0,4,8,24 h) and different diluents (the original、formula Ⅰ and formula Ⅱ) on sperm motility and the results of artificial insemination in low-temperature (4 ℃) storage were studied in this trial.192 Huang hens of 33 weeks old were selected and divided into 12 groups as (3 sorts of processed semen×4 storage time) with 16 hens for each,42 Huang cocks of 33 weeks old were selected as one group.After collecting semen,two kinds of diluents was used to dilute semen and kept them in 4 ℃ for 0,4,8,24 hours,then the sperm motility and inseminated were observed,eggs were collected from all groups for measurement of the fertilization rate,birth rate and healthy chicks rate of each group.The experiment was taken with the original semen kept in 4 ℃ as control.The results showed that sperm motility of the two dilute semen was extremely significantly higher than that of the semen group (P<0.01) and the sperm motility of formula Ⅱ group was higher than that of formula Ⅰ group (P>0.05) after storage of 4,8 and 24 h.Fertilization rates of the two dilute semen were significantly higher than that of the semen group (P<0.05) after storage of 4 h;Fertilization rates of the two dilute semen were extremely significantly higher than that of the semen group (P<0.01) after storage of 8 h.The two semen diluents were more effective for semen preservation,and the two dilute semen could significantly improve the fertilization rate.The result provided a powerful support for the improvement of semen preservation skills in chicken farms in Southern China.

Key words: semen diluent; sperm motility; fertilization rate; birth rate; healthy chicks rate

CLC Number:

S831

ZHOU Lele, LI Chengde, HUANG Jianhao, CHEN Yun, ZENG Fanwen, HUANG Xiaogang, ZHANG Faliang, ZHENG Xican, YAN Changyan, WEI Hengxi, ZHANG Shouquan. Effects of Different Diluents on Sperm Motility and Insemination Results of Chicken Semen in Low-temperature Storage[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1837-1843.

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Effects of Different Diluents on Sperm Motility and Insemination Results of Chicken Semen in Low-temperature Storage

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