›› 2010, Vol. 37 ›› Issue (4): 146-149.

• 遗传繁育 • Previous Articles     Next Articles

Optimization of Goat Embryos in vitro Culture Conditions

LIU Yi-yi1，ZHAO Gao-ping2，LI Yan4，BAI Jun2， WANG Shen-yuan2，BAR Ter2，ZHOU Huan-min3   

  1. （1.College of Biotechnology, Inner Mongolia Agricultural University, Hohhot 010018, China;2.College of Animal Science and Medical Science, Inner Mongolia Agricultural University, Hohhot 010018, China；3.Science and Technology Agency, Inner Mongolia Agricultural University, Hohhot 010018, China;4.The Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot 010021, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20

Abstract: This experiment compared the effect of mature liquid that containing three different sources serum: high-quality fetal bovine serum, estrus goat serum, PG treated estrus goat serum, maturation rate were 65.95%, 49.2%, 76.47% （P＜0.01）respectively, the difference was extremely significant; compared SOFaa, CR1aa+ oviduct epithelial cells, and improved DMEM/F12 development system and found that, the blastocyst rate of SOFaa, CR1aa+ oviduct epithelial cells were 29.62%, 24.73%, difference was not significant(P＞0.05), the DMEM/F12 system had higher blastocyst development rates 48.42%, the difference was very significant（P＜0.01）.

Key words: estrus goat serum; blastocyst rate; oviduct epithelial cells

CLC Number: