›› 2010, Vol. 37 ›› Issue (2): 218-220.

• 经验交流 • Previous Articles     Next Articles

Isolation and Identification of Haemophilus parasuis

LI Xiao-hua, YANG Xiao-yan, ZHENG Xin-tian, DAI Ai-ling, LI Gai-na   

  1. (College of Life Sciences of Longyan University, Key Laboratory of Preventive Veterinary Medicine and Biotechnology, Engineering Research Center for the Prevention and Control of Zoonosis, Longyan 364000, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

Abstract: Abstract:A covey of swine whose clinical symptoms looked like Haemophilus parasuis disease were checked by lab technologies. Research as bacteria observation, cultural characteristics and biochemical properties of Haemophilus parasuis were done. According to the sequence of bacterium 16S rRNA, special primers were designed for PCR amplification. The results showed that the strain was tentatively identified as Haemophilus parasuis. The drug sensitive tests showed:the strain was sensitive to Ciprolfloxacin, Kanamycin, Doxycycline, Ampicillin, Amikacin, Gentamicin; but resistant to Tiamulin, Norfloxacin.

Key words: Key words:Haemophilus parasuis; isolation and identification; drug sensitive test